Molecular characterization and antimicrobial resistance of bacterial pathogens in neonatal sepsis: A cross-sectional study at a Lagos tertiary hospital

Egwuatu, Tenny Obiageli Gladys; Akingboye, Desmond Wemimo; Ottairoegbu, Benedicta; Lawal, Tolani Simibat; Adeyemo, Nimota Ajibike; Ajayi, Abraham; Essiet, Utibeima Udo; Oladetuyi, Peter Adedayo; Lesi, Afolabi; Smith, Stella Ifeanyi

doi:10.21608/mid.2024.306324.2095

	Molecular characterization and antimicrobial resistance of bacterial pathogens in neonatal sepsis: A cross-sectional study at a Lagos tertiary hospital
Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 27 October 2024
Document Type: Original Article
DOI: 10.21608/mid.2024.306324.2095
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Authors
Tenny Obiageli Gladys Egwuatu ¹; Desmond Wemimo Akingboye¹; Benedicta Ottairoegbu²; Tolani Simibat Lawal²; Nimota Ajibike Adeyemo³; Abraham Ajayi ²; Utibeima Udo Essiet ²; Peter Adedayo Oladetuyi⁴; Afolabi Lesi ³; Stella Ifeanyi Smith ²^{, 5}
¹Department of Microbiology, University of Lagos Akoka, Nigeria
²Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
³Department of Paediatrics, College of Medicine, University of Lagos Idi-Araba, Lagos Nigeria
⁴Department of Science Laboratory Technology, Eko-College of Management and Technology, Ikotun, Lagos, Nigeria
⁵Department of Biological Sciences, Mountain Top University, Ogun State, Nigeria
Abstract
Background: Neonatal sepsis, a high-risk disease, is a major contributory factor to infant morbidity and mortality in low- and middle-income countries. The aim of this study was to isolate and molecularly characterize bacterial pathogens causing sepsis in neonates. Methods: This study characterized and evaluated the antimicrobial susceptibility profile of the bacterial aetiology of neonatal sepsis in the neonatal ward of a tertiary hospital in Lagos. Ninety-five blood samples were collected from neonates presenting with sepsis. Microbiology and molecular biology methods were used for the identification and characterization of bacterial isolates, while antibiotic susceptibility testing was conducted using the Kirby-Bauer disc diffusion method. Results: Of the participants, 67.4% were between 1 day and 1 week old, and 86.3% had an onset of temperature between 1 day and 5 days. Twenty percent of the blood samples were culture positive, revealing 32% Gram-positive bacteria and 60% Gram-negative bacteria. There was no significant difference (p = 0.8374) in the rate of positive blood cultures between male and female participants. The dominant bacterial species identified were Enterobacter quasihormaechei and Enterobacter cancerogenus. Other isolated species included Bacillus aerius, Bacillus inaquosorum, Bacillus paramycoides, Klebsiella pneumoniae, Klebsiella variicola, Staphylococcus hominis, Staphylococcus saccharolyticus, and Cedecea colo. Notably, 95% of the isolates were resistant to cefuroxime, 89% to cefepime, and 74% to amoxicillin + clavulanic acid. Two isolates, AH-1 (Enterobacter roggenkampii) and ON-2 (Cedecea colo), were resistant to eight of the nine tested antibiotics. Conclusion: The high rate of multidrug-resistant bacterial strains implicated in neonatal sepsis underscores the need for routine surveillance and improved hygiene practices in neonatal wards.
Keywords
Antibiotic resistance; Bacteria; Hospital; Neonates; Sepsis


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