Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia
El-Awady, M., Abdel-Hady, M., Hegazy, A., Samy, D., Rashad, H., Shahat, H., Mohamed, M., Samha, M., Salah, E. (2024). Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia. EKB Journal Management System, 67(13), 1863-1871. doi: 10.21608/ejchem.2024.316633.10318
Mohamed A. M. El-Awady; M. S. Abdel-Hady; Alia M. Hegazy; Dina H. Samy; Habibatullah H. Rashad; Habiba Shahat; Mayar W. Mohamed; Minas K. Samha; Esmaeel M. Salah. "Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia". EKB Journal Management System, 67, 13, 2024, 1863-1871. doi: 10.21608/ejchem.2024.316633.10318
El-Awady, M., Abdel-Hady, M., Hegazy, A., Samy, D., Rashad, H., Shahat, H., Mohamed, M., Samha, M., Salah, E. (2024). 'Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia', EKB Journal Management System, 67(13), pp. 1863-1871. doi: 10.21608/ejchem.2024.316633.10318
El-Awady, M., Abdel-Hady, M., Hegazy, A., Samy, D., Rashad, H., Shahat, H., Mohamed, M., Samha, M., Salah, E. Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia. EKB Journal Management System, 2024; 67(13): 1863-1871. doi: 10.21608/ejchem.2024.316633.10318

Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia

Egyptian Journal of Chemistry
Volume 67, Issue 13, December 2024, Pages 1863-1871    PDF (620.29 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.316633.10318
Authors
Mohamed A. M. El-Awady1; M. S. Abdel-Hady2; Alia M. Hegazy3; Dina H. Samy3; Habibatullah H. Rashad3; Habiba Shahat3; Mayar W. Mohamed3; Minas K. Samha3; Esmaeel M. Salah* 4
1Genetics Department, College of Agriculture, Cairo University, Giza, Egypt.
2Biotechnology Group, Botany Dept., National Research Centre, Dokki, Giza, Egypt.
3Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.
4Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo.
Abstract
Datura innoxia is renowned for its pharmaceutical qualities and traditional medical use. It’s crucial for long-term pharmaceutical drug synthesis by regulated micropropagation and tissue culture techniques. This study focused on establishing Datura innoxia under aseptic laboratory settings to promote callus formation and comparing its phytochemical profile with leaves using High-Performance Liquid Chromatography (HPLC). The goal was to identify potential changes in phytochemical content between various growing strategies. Antibacterial efficacy of extracts from conventional plants and lab-grown callus were evaluated against Escherichia coli and Staphylococcus aureus strains. Moreover, aiming to ascertain the genetic stability of Datura innoxia cells in different conditions, the fingerprinting profile was performed by Start Codon Targeted (SCoT) and Inter-Simple Sequence Repeat (ISSR) markers. By thoroughly examining these features, the study provides insights into the biochemical, pharmacological, and genetic properties of Datura innoxia, which are critical for its use in pharmaceutical research and development. These findings highlight the critical role of regulated cultivation practices in improving the efficacy and sustainability of medicinal plant-based therapies.
Keywords
Datura innoxia; Tissue Culture; HPLC; Antimicrobial Activity; SCoT; ISSR; Fingerprinting
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