RETN gene polymorphisms and serum resistin levels in patients with breast cancer | ||||
| Egyptian Journal of Cancer and Biomedical Research | ||||
| Volume 8, Issue 4, December 2024, Page 1-10 PDF (590.7 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcbr.2024.304343.1361 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sarah M. Shoeib1; Radwa Mahmoud Elsharaby1; Sara Elakshar2; Rehab M El-Gohary3; Yosra Abdelmonem Zamzam   1
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| 1Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt | ||||
| 2Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Tanta University, Egypt | ||||
| 3Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt | ||||
| Abstract | ||||
| Background: Increased serum resisitin levels act as an effective pro-inflammatory mediator, which may play a key role in cancer progression and promotion and may be caused by RETN gene polymorphisms. Aim of the work: We investigated serum resistin levels in breast cancer patients as well as the relationship between the RETN single nucleotide polymorphisms (rs7408174 and rs3219175) and breast cancer susceptibility. Subjects and Methods: This case control study included 80 breast cancer patients and 80 healthy controls. Genotyping of RETN rs7408174 and rs3219175 gene polymorphism were determined by real time polymerase chain reaction (RT-PCR) TaqMan allelic discrimination assay. Serum resistin levels were quantified using Enzyme-linked immunosorbent assay (ELISA). Results: Breast cancer patients had higher serum resistin levels compared to healthy controls. Patients with rs3219175 AG and rs7408174 CT/CC genotypes had significantly higher serum levels of resistin. Carriers of the single nucleotide polymorphism (SNP) rs3219175 heterozygous (AG) and homozygous (AA) genotypes had a significantly higher breast cancer risk than carriers of the wild (GG) genotype (Odd ratio (OR): 2.437; 95% CI: 1.262-4.707 and 3.091; 0.723-13.222, respectively). Patients carrying rs3219175 AG/AG+AA and rs7408174 CT/CC genotypes had a higher risk of developing advanced tumour size (T3+T4), lymph node (LN) infiltration, and advanced TNM stage (III+IV). Conclusion: There is a strong link between RETN SNP rs3219175 and high serum resistin levels and the risk of breast cancer. Furthermore, the RETN SNPs rs3219175 and rs7408174 are linked to poor clinicopathological status. | ||||
| Keywords | ||||
| Key words: Breast cancer; Resistin; RETN gene polymorphism | ||||
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