Antifungal activity of biosynthesized silver nanoparticles against fluconazole-resistant Candida isolated from some Egyptian hospitals. | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 16 November 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.332476.2322 | ||||
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| Authors | ||||
Islam A. Aldesoukey  1; Marwa Mostafa Abdul-Aziz2
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| 1Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt | ||||
| 2The Regional Centre for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Fungal infections pose a significant global healthcare challenge, particularly due to the rising incidence of such infections in recent decades. This increase is notably pronounced among immunocompromised individuals, who are more susceptible to developing fungal sepsis. The present study aims to estimate the antifungal effectiveness of biosynthesized silver nanoparticles (Ag NPs) against drug-resistant strains of Candida. Materials and Methods: This research investigates Twelve Candida isolates were identified using standard microbiological methods and chrome agar. Antifungal resistance was evaluated through disk diffusion and MIC analysis. Ag NPs were synthesized using a green approach and characterized via U.V spectroscopy, SEM, EDX, XRD, HRTEM, Zeta potential, and FTIR analysis. Antifungal effect of Ag NPs was assessed, along with their synergistic effects when combined with fluconazole, against multidrug-resistant Candida isolates. Results: Twelve Candida isolates were Candida albicans (four), Candida parapsilosis (six), Candida glabrata (one), and Candida tropicalis (one). Susceptibility patterns of the isolates revealed that all strains were resistant to fluconazole (100%), followed by miconazole (92%) and voriconazole (58%). In contrast, a high sensitivity was observed towards posaconazole, with only 8% of the isolates exhibiting resistance, the optical properties of AgNPs were analyzed using UV-Visible spectrophotometry, while SEM, EDX, and XRD assessed surface characteristics; FTIR revealed function groups, zeta potential assessed chanrge and HRTEM determined particle size and shape. AgNPs displayed significant antimicrobial activity against the majority of Candida isolates. Conclusion: When Ag NPs were combined with fluconazole, 50% of the isolates (6/12) demonstrated a synergistic effect, while the remaining 50% showed an additive effect. | ||||
| Keywords | ||||
| Candida; Fluconazole-Resistant; Silver nanoparticles | ||||
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