In vitro Effect of Vitamin D on the Levels of Cytokines and Apoptosis Related Markers in Systemic Lupus Erythematosus
Al-Noman, H., Salama, L., Fawzy, I., Elzayady, M., Mahmoud, A., Mesbah, M., Elageery, S., Alkasaby, N., Badr, D. (2025). In vitro Effect of Vitamin D on the Levels of Cytokines and Apoptosis Related Markers in Systemic Lupus Erythematosus. EKB Journal Management System, 34(1), 219-255. doi: 10.21608/ejmm.2024.335543.1369
Hifaa G. Al-Noman; Lamiaa A. Salama; Iman M. Fawzy; Mohamed M. Elzayady; Abeer A. Mahmoud; Maggie R. Mesbah; Safaa Mohamad Elageery; Nashwa M. Alkasaby; Dina F. Badr. "In vitro Effect of Vitamin D on the Levels of Cytokines and Apoptosis Related Markers in Systemic Lupus Erythematosus". EKB Journal Management System, 34, 1, 2025, 219-255. doi: 10.21608/ejmm.2024.335543.1369
Al-Noman, H., Salama, L., Fawzy, I., Elzayady, M., Mahmoud, A., Mesbah, M., Elageery, S., Alkasaby, N., Badr, D. (2025). 'In vitro Effect of Vitamin D on the Levels of Cytokines and Apoptosis Related Markers in Systemic Lupus Erythematosus', EKB Journal Management System, 34(1), pp. 219-255. doi: 10.21608/ejmm.2024.335543.1369
Al-Noman, H., Salama, L., Fawzy, I., Elzayady, M., Mahmoud, A., Mesbah, M., Elageery, S., Alkasaby, N., Badr, D. In vitro Effect of Vitamin D on the Levels of Cytokines and Apoptosis Related Markers in Systemic Lupus Erythematosus. EKB Journal Management System, 2025; 34(1): 219-255. doi: 10.21608/ejmm.2024.335543.1369

In vitro Effect of Vitamin D on the Levels of Cytokines and Apoptosis Related Markers in Systemic Lupus Erythematosus

Egyptian Journal of Medical Microbiology
Article 25, Volume 34, Issue 1, January 2025, Page 219-255  XML PDF (435.39 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.335543.1369
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Authors
Hifaa G. Al-Noman1; Lamiaa A. Salama2; Iman M. Fawzy3; Mohamed M. Elzayady4; Abeer A. Mahmoud4; Maggie R. Mesbah5; Safaa Mohamad Elageery email 5; Nashwa M. Alkasaby5; Dina F. Badr5
1Nogoud Medical Center, Almadinah Almonawarah, Ministry of Health, KSA
2Microbiology and Immunology Department, Faculty of Pharmacy, Horus University, Egypt
3Mansoura Central Laboratories, Clinical Pathology Department, Ministry of Health, Egypt
4Specialized Medical Hospital, Faculty of Medicine, Mansoura University
5Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt
Abstract
Abstract
Background: Both genetic and environmental factors are responsible for systemic lupus erythematosus (SLE) pathogenesis. knowledge about the roles of cytokines and apoptosis in SLE have led to new clinical points of view in its pathogenesis. Objective: This work aimed to study the in vitro effects of vitamin D3 on the level of IL-6, TNF-α, INF-γ, and IL-10 cytokines, as well as the expression of Bax and Bcl-2 in peripheral blood mononuclear cells (PBMCs) cultures of SLE patients. Methods: Isolated PBMCs from 50 SLE patients were cultured with 1,25(OH)2D3. Vitamin D treated cultured cells were assessed for detection of the levels of cytokines by ELISA and gene expression of apoptotic markers by real time-quantitative reverse transcription PCR (RT-qPCR). Results: It was found that vitamin D significantly decreased the levels of IL-6, TNF -α, IFN-γ and IL-10 in vitamin D treated cells of SLE patients relative to the untreated cells (P value<0.001 for each). Vtamin D3 treated cells showed that expressions of Bax (0.67±0.12) and Bcl‐2 (19.83±6.6) underwent fold change compared to that in untreated cells. It was found downregulation of Bax expression (3.5%) and upregulation of Bcl‐2 expression (1.7 fold increase) in vitamin D treated cells. Conclusion: Vitamin D is an immunomodulator agent and its administration in SLE patients may exert a good therapeutic effect. It had both inhibitory effect on cytokine levels and antiapoptotic effect in PBMCs of SLE patients.
Keywords
Vitamin D; SLE; cytokines; apoptosis
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