Assessment of Protective Effect of Rutin Utilizing Advanced Intranasal Nanoparticulate System in Mice Model after Experimental Challenge with Pasteurella Multocida Isolated from Naturally Infected Sheep | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Article 16, Volume 56, Issue 8, August 2025, Page 1805-1822 PDF (1.27 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2024.297980.2183 | ||||
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| Authors | ||||
Iman AM Abdel-Rahman1; Amany M. Mohamed 2; Nagwa I. toaleb 2; Ahmad M. Allam 2; Sherein S. Abdelgayed3; Abeer Saad Hassan  4; Sahar Hussein abdalla hekal5
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| 1Department of Pharmacognosy, Faculty of Pharmacy, South Valley University, Qena 83523, Egypt. | ||||
| 2Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 Bohouth St., Dokki, 12622 Giza, Egypt. | ||||
| 3Pathobiology department, College of Veterinary Medicine, Tuskegee University, AL36808, USA. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt. | ||||
| 4Department of Pharmaceutics-Faculty of Pharmacy- South Valley University- Qena-Egypt | ||||
| 5Department of Natural Resources Faculty of African Postgraduate Studies, Cairo University, Giza, Egypt. | ||||
| Abstract | ||||
| Rutin is a promising nutraceutical with potential antibacterial and prophylactic activities. This work aimed to evaluate the protective effect of Rutin nanovehicle, which were formulated as a mucoadhesive intranasal preparation, on mice experimentally challenged with Pasteurella multocida. The extracted Rutin nanoplatform was laden into different concentrations of mucoadhesive hydroxy propyl methyl cellulose (HPMC). The ex vivo permeability of Rutin nanoformulations across the nasal mucosa was evaluated. The optimal formulation was examined in vivo, and group 1 was not infected or prophylactic. Group 2 was infected with P. multocida-nonprotected, and groups 3 and 4 were prophylactic groups that were protected with free Rutin extract and Rutin nanoformulation, respectively, via the intranasal route. The bacterial count in vital organs was carried out post challenge with P. multocida to determine protective effect of free Rutin extract and Rutin nanoformulation, also pathological changes in the vital organs were examine, level of IgG was determined, Cytokines concentration; Interferon Gamma (INF-γ) and Interlukin-12 (IL -12) was assessment by using sandwich ELISA. The rutin nanoparticulate system containing the lowest amount of HPMC exhibited enhanced permeated amount (311.64± 10.0 μg/cm2) and steady state flux 6.67± 0.10 μg /cm 2/sec across nasal mucosa. The results of the in vivo study revealed that the rutin nanoformulation showed a significant reduction in the bacterial count, histopathological lesions in the examined organs. Also, increased the hormonal response of mice where it increased levels of antibodies IgGs and modulation in the concentration of serum IFN-γ and IL-12. The mucoadhesive intranasal Rutin nanovehicle has remarkable immunomodulatory effects and can be used for protection against Pasteurella multocida in animals. | ||||
| Keywords | ||||
| Pasteurella multocida; prophylaxis; free Rutin extract; Rutin nanostructure lipid carrier | ||||
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