Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa

Ghania, Asmaa; Abdel-Ghaffar, Alaa Elsayed; Sosa, Gamal Abdel-raheem Mohamed; Badr, Magdy Ramadan; Agag, Mohsen Abd Elhafeez; Kandiel, Mohamed Mahmoud M.; Sakr, Abdelaziz M.; Kortam, Laila E.

doi:10.21608/javs.2024.323359.1427

	Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa
Journal of Applied Veterinary Sciences
Article 2, Volume 10, Issue 1, January 2025, Pages 10-21 PDF (479.17 K)
Document Type: Original Article
DOI: 10.21608/javs.2024.323359.1427
Authors
Asmaa Ghania^* ¹; Alaa Elsayed Abdel-Ghaffar²; Gamal Abdel-raheem Mohamed Sosa¹; Magdy Ramadan Badr³; Mohsen Abd Elhafeez Agag⁴; Mohamed Mahmoud M. Kandiel¹; Abdelaziz M. Sakr⁵; Laila E. Kortam⁶
¹Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
²Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Delta University for Science and Technology, Gamasa, Dakahlia, Egypt.
³Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 12556 Haram, Giza, Egypt.
⁴Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
⁵Animal Production Research Institute, Agricultural Research Center (A RC), Dokki, Giza, Egypt
⁶Immunity Unit, Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 12556 Haram, Giza, Egypt
Abstract
This study aimed to examine how varying concentrations (0.5%, 1.0%, 2.0%, 3.0%, and 4.0%) of nano-L-α phosphatidylcholine (nano-L-α-PC) affected the quality of post-thawed buffalo bull semen compared to the same concentrations of L-α-phosphatidylcholine (L-α-PC), while 20% egg yolk (EY) was used as a control. Ejaculates (n = 72) were collected from fertile buffalo bulls (n = 12) for 6 weeks. The ejaculates were pooled and divided into 11 groups (3 aliquots per group) extended with 20% EY (Group 1 without supplement), 0.5%, 1.0%, 2.0%, 3.0%, and 4.0% nano-L-α-PC (Groups 2–6), and 0.5%, 1.0%, 2.0%, 3.0%, and 4.0% L-α-PC (Groups 7–11) in tris buffer. After cryopreservation procedures, semen samples were thawed at 37 °C and then evaluated for sperm kinematics, acrosomes, plasma membranes, and DNA integrities. The seminal plasma was analyzed for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione reduced (GSH), and catalase (CAT). Results demonstrated that extended semen samples containing 1.0-3.0% nano-L-α-PC and 1.0-4.0% L-α-PC showed high total motility compared to control, whereas at 0.5–4.0% of nano-L-α-PC and L-α-PC showed high progressive motility. Significant (P< 0.05) wobble levels were seen at 0.5, 1.0, and 3.0% nano-L-α-PC, and distance average path and velocity average path at 2.0% L-α-PC. Acrosome and plasma membrane integrities were markedly elevated (P< 0.0001) at 2.0% nano-L-α-PC and 4.0% L-α-PC. High DNA integrity metrics were noticed at 0.5–1.0% nano-L-α-PC, as well as 3.0% and 4% L-α-PC. Low MDA and high (SOD, GSH, and CAT) levels were observed at 2.0% nano-L-α-PC and 4.0% L-α-PC. In conclusion, the optimum concentration of nano-L-α-PC that improved semen quality was 2.0%, which is roughly equivalent to the effect of 4.0% L-α-PC.
Keywords
Antioxidant capacity; Bull semen; Comet assay; Cryopreservation; Nano soy lecithin

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