Role of Moringa Oleifera Leaf Extract in alleviating cerebellar ataxia induced by monosodium glutamate in rodent model

Kiwan, Nedaa; Askar, Mona H.; Shaker, Gehan A.; Othman, Basma H.; El Menanawy, Fayza R.

doi:10.21608/besps.2024.319520.1176

	Role of Moringa Oleifera Leaf Extract in alleviating cerebellar ataxia induced by monosodium glutamate in rodent model
Bulletin of Egyptian Society for Physiological Sciences
Volume 45, Issue 1, January 2025, Page 77-94 PDF (987.94 K)
Document Type: Review Article
DOI: 10.21608/besps.2024.319520.1176
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Authors
Nedaa Kiwan ¹; Mona H. Askar ²; Gehan A. Shaker²; Basma H. Othman³; Fayza R. El Menanawy²
¹Medical physiology department, faculty of medicine, Mansoura University, Mansoura, Egypt.
²Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt
³Mansoura medical experimental research center MERC, Faculty of medicine, Mansoura University, Egypt
Abstract
Objective: Studying the antioxidant properties of Moringa oleifera (MO) leaf extract as a prevention and treatment therapy on monosodium glutamate (MSG)-induced cerebellar ataxia rat model and investigating the role of Nrf2-Keap1 pathway. Methods: Thirty male Sprague-Dawley rats were allocated into four groups. Control group: six rats were administered distilled water orally for ten days. MSG group: eight rats received 4 g/kg bw of MSG orally for ten days. MSG + MO prevention group: eight rats received oral MSG 4 g/kg bw for ten days and 400 mg/kg bw of MO leaf extract orally for 21 days. MSG + MO treatment group: eight rats received 4 g/kg bw MSG orally for ten days, then 400 mg/kg bw of MO leaf extract orally for 21 days. We conducted basal and motor behavioral assessments. We carried out a histopathological inspection and a biochemical analysis to measure oxidative stress markers MDA and GSH levels in the cerebellar tissue. We also conducted an immunohistochemical analysis to evaluate the nuclear expression of Nrf2. Results: MO prevention and treatment therapy increased some motor behavior parameters. Both increased Purkinje cell survival (P <0.01), GSH levels, and nuclear Nrf2 expression. MO prevention therapy decreased MDA levels compared to MSG P <0.01. Conclusions: MO prevention and treatment therapy have a possible neuroprotective effect on MSG-induced cerebellar ataxia rat model through the Nrf2-Keap1 pathway. Still, MO prevention therapy may have the upper hand over treatment therapy.
Keywords
cerebellar ataxia; Moringa oleifera; monosodium glutamate; oxidative stress; Nrf2


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