GELATIN AS A HIGH VISCOSITY PROTEIN IN TRIS EXTENDER AND ITS EFFECT ON POST-THAWED RAM SEMEN QUALITY | ||||
| Egyptian Journal of Applied Science | ||||
| Volume 39, Issue 11, November 2024, Page 63-74 PDF (467.04 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejas.2024.393644 | ||||
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| Abstract | ||||
| ABSTRACT Gelatin as a high viscosity protein can reduce sperm metabolism by reducing sperm movement. So, this study was set out to examine the impact of adding gelatin to Tris-citric-egg yolk extender (0, 0.5, 1.0 and 1.5%) on visual sperm characteristics, CASA analysis parameters, total antioxidants capacity, and enzyme activity in in post-thawed ram semen. Total of 5 rams have been used for collecting semen once weekly for 5 weeks. Pooled semen was diluted with the different levels of gelatin, equilibrated at 5oC for 4h and stored in liquid nitrogen. Semen had been evaluated post thawing. Results showed a positive effect of gelatin supplementation in extender on sperm characteristics, including progressive motility, livability, abnormality, and membrane integrity. Gelatin addition improved CASA -motility parameters and normality, while decreasing sperm velocity of sperm cells as well as increasing total antioxidant capacity and glutathione peroxidase, and decreasing enzyme activity (LDH) of post-thawed sperm medium. In conclusion, gelatin supplementation to Tris semen extender at a level of 1.5% is considered as a good enhancement on sperm function in post-thawed ram semen. | ||||
| Keywords | ||||
| Key Words: Gelatin; Ram semen; Computer assisted sperm analysis (CASA) | ||||
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