Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria
LAWAL, A., Kabir, L., Balarabe, F., Abubakar, Y., Usman, M., Lawal, F., Umar, Y. (2025). Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria. EKB Journal Management System, 6(3), 5020-5028. doi: 10.21608/mid.2024.323848.2248
ABDULRAZAK LAWAL; Lawan Muhammad Kabir; Fatima Tanimu Balarabe; Yusuf Abubakar; Mannir Dahiru Usman; Fatima Yusuf Lawal; Yuguda Muhammad Umar. "Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria". EKB Journal Management System, 6, 3, 2025, 5020-5028. doi: 10.21608/mid.2024.323848.2248
LAWAL, A., Kabir, L., Balarabe, F., Abubakar, Y., Usman, M., Lawal, F., Umar, Y. (2025). 'Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria', EKB Journal Management System, 6(3), pp. 5020-5028. doi: 10.21608/mid.2024.323848.2248
LAWAL, A., Kabir, L., Balarabe, F., Abubakar, Y., Usman, M., Lawal, F., Umar, Y. Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria. EKB Journal Management System, 2025; 6(3): 5020-5028. doi: 10.21608/mid.2024.323848.2248

Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria

Microbes and Infectious Diseases
Volume 6, Issue 3, August 2025, Page 5020-5028  XML PDF (1.02 MB)
Document Type: Original Article
DOI: 10.21608/mid.2024.323848.2248
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Authors
ABDULRAZAK LAWAL email orcid 1; Lawan Muhammad Kabir2; Fatima Tanimu Balarabeorcid 3; Yusuf Abubakarorcid 4; Mannir Dahiru Usmanorcid 5; Fatima Yusuf Lawalorcid 2; Yuguda Muhammad Umar1
1Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Bayero University Kano, Nigeria.
2Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University Zaria, Nigeria.
3Department of scientific and industrial research, National Research Institute For Chemical Technology Zaria, Nigeria.
4Federal University of Agriculture Zuru, Zuru, Kebbi State, Nigeria
5Faculty of Veterinary Medicine, Bayero University, Kano Nigeria
Abstract
Background: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a significant zoonotic pathogen implicated in severe human illnesses such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). Cattle are known asymptomatic reservoirs, and their fecal shedding of the pathogen poses a public health risk through contamination of food and water sources. This study aimed to isolate and molecularly characterize STEC O157:H7 from cattle feces within Zaria Metropolis, Nigeria. Methods: A cross sectional study was conducted. A total of 328 cattle faecal samples were collected from Eight (8) different Fulani cattle herds. Faecal samples where enriched, selectively cultured, and screened for E. coli O157:H7 using Wellcolex rapid latex agglutination kit and multiplex polymerase chain reaction (PCR) to detect stx1, stx2, eaeA and hlyA genes. Results: A total of 26 (7.93%) isolates were positive for E. coli O157:H7 using Wellcolex rapid latex agglutination kit. Out of which, 1(0.31%) isolate was found in bull while 25(7.62%) were in cows, 3(0.91%) isolates were in calves while 23(7.02%) in adult cattle and 4(1.22%) were in Bokoloji while 22(6.71%) were in Bunaji, a total 26 isolates were subjected to multiplex PCR, only 2(7.69%) expressed the shiga toxin genes (stx1). Conclusion: This study highlights the presence of shiga toxin producing E. coli O157:H7 in the cattle population of Zaria and the need for stringent control measures to prevent transmission to humans.
Keywords
Escherichia coli O157:H7; Shiga toxin; zoonosis; Latex agglutination; Multiplex PCR
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