Combating Increased Oxidative Stress and Hepatic Injury Induced by CCl4 in Wistar Rats by A Polyphenol Rich Jam from Edible Plants | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 68, Issue 7, July 2025, Page 339-349 PDF (4.55 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.329059.10647 | ||||
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| Authors | ||||
Maha Hanafy Mahmoud   1; Hany Mohamed Ahmed Wahba 2; Marwa Hanafy Mahmoud 3
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| 1Nutrition and Food Science Dept., Institute of Food Industries and Nutrition, National Research Centre, Cairo, Egypt. | ||||
| 2Nutrition and Food Science Dept., Institute of Food Industries and Nutrition, National Research Centre, Cairo, Egypt. | ||||
| 3Food Technology Dept., Institute of Food Industries and Nutrition, National Research Centre, Cairo, Egypt | ||||
| Abstract | ||||
| Oxidative stress has been regarded as an etiologic factor for many degenerative and chronic diseases. Carbon tetrachloride (CCl4) is a strong oxidizing agent that is used in many purposes and many dangerous and hazardous effects accompany its use. Use of natural antioxidants like edible plants can combat the deleterious effects of the oxidizing compounds. The present study aims to investigate the use of a mixture of potent antioxidant edible plants to mitigate the hazardous effects of CCl4. The formulated edible plant mixture comprising; sweet corn, pomegranate peel, germinated soyabean, raisins, sumac, dried black plum, golden berry, carrots and chicory was evaluated for its polyphenol and flavonoid content and was used to produce a jam whose sensory evaluation was tested. It was found to contain a relatively high polyphenol and flavonoid content. A model of increased oxidative stress with hepatic injury in Wistar rats was achieved by CCl4 injection and the ameliorative impact of the formulated mixture of plants was evaluated. Five groups were included each of seven rats. Altered biochemical parameters resulting from CCl4 injection includes, an elevation in serum MDA and glucose concentrations, AST and ALT activities and TNF-α concentration along with a decline in body weight, total protein, albumin, hemoglobin along with a change in the enzymatic antioxidant profile with altered histopathological examination. The formulated plant mixture with its two tested concentrations 10 and 20% was able to restore all altered parameters and histopathological findings to a good extent in a dose dependent manner. It can be concluded that all features of increased oxidative stress and hepatic injury obtained by CCl4 injection were restored by the prepared formula. It is highly recommended to use the produced jam from the edible plant mixture as a dietary supplement for those persons that are highly exposed to increased oxidative stress due to CCl4 or any other oxidizing compounds. | ||||
| Keywords | ||||
| oxidative stress. CCl4; hepatic injury; edible plants; polyphenols; flavonoids | ||||
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