Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method
Mahgoub, Y., El-Hawiet, A., El Sebakhy, N., Darwish, F., Shawky, E. (2025). Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method. EKB Journal Management System, 2(1), 17-23. doi: 10.21608/japs.2024.290405.1027
Yasmin Adel Mahgoub; Amr Moustafa El-Hawiet; Nadia Abdel Aziz El Sebakhy; Fikria Ali Darwish; Eman Shawky. "Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method". EKB Journal Management System, 2, 1, 2025, 17-23. doi: 10.21608/japs.2024.290405.1027
Mahgoub, Y., El-Hawiet, A., El Sebakhy, N., Darwish, F., Shawky, E. (2025). 'Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method', EKB Journal Management System, 2(1), pp. 17-23. doi: 10.21608/japs.2024.290405.1027
Mahgoub, Y., El-Hawiet, A., El Sebakhy, N., Darwish, F., Shawky, E. Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method. EKB Journal Management System, 2025; 2(1): 17-23. doi: 10.21608/japs.2024.290405.1027

Tracking and quantifying the major silymarin flavonolignans in the fruit extracts of milk thistle (Silybum marianum L.) using a validated HPTLC-MS method

Journal of Advanced Pharmaceutical Sciences
Volume 2, Issue 1, January 2025, Pages 17-23    PDF (617.85 K)
Document Type: Short Communication
DOI: 10.21608/japs.2024.290405.1027
Authors
Yasmin Adel Mahgoub* ; Amr Moustafa El-Hawiet; Nadia Abdel Aziz El Sebakhy; Fikria Ali Darwish; Eman Shawky
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
Abstract
High performance thin layer chromatography hyphenated with mass spectrometry (HPTLC-MS) was implemented for the first time for the simultaneous tracking and quantitation of the major flavonolignans in fruit extracts of milk thistle (Silybum marianum L.) varieties; marianum and albiflora.  A mobile phase of chloroform: acetone: formic acid (110: 16.5: 8.5 v/v/v) was utilized for the development of the tracked flavonolignans followed by their visualization under UV 366 nm after NP/PEG derivatization. The tracked flavonolignans were identified, using mass analysis of the developed flavonolignans directly from the developed plate, and quantified. The developed method's linearity, accuracy, and precision were all confirmed in accordance with ICH requirements. Linear calibrations for the quantified flavonolignans were obtained with a correlation coefficient > 0.98. Precision was confirmed with a relative standard deviation ranging between 1.34 and 1.83%.  In addition, acceptable accuracy was obtained with recovery exceeding 97%, as well as appropriate limit of detection (LOD) and limit of quantitation (LOQ).
 
Keywords
HPTLC-MS; Silybum varieties; Flavonolignans
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