The possible protective role of vitamin E on adrenal cortex of male albino rats exposed to heat stress: a histo-biochemical study. | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 December 2024 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.324567.2149 | ||
| Authors | ||
| Amany Elsayed Hamoud* 1; Doaa Mahmoud Shuaib2; Maryam Atef Fahim3; Ahmed Galal Motawie4; Hany Abdel-latif Soliman5; Dina Mohamed Hussein6 | ||
| 1Faculty of medicine Cairo University Egypt Kasrelaini | ||
| 2Anatomy department,faculty of medicine cairo university. | ||
| 3Anatomy department, Faculty of medicine Cairo University | ||
| 4Anatomy department, Faculty of medicine Cairo university | ||
| 5Anatomy department, Faculty of medicine, Cairo university | ||
| 6Anatomy department, Faculty of medicine, Cairo university | ||
| Abstract | ||
| Background: Heat stress can affect the adrenal glands in different ways. The current research aimed to evaluate the possible prophylactic role of vitamin E against the negative impact of acute heat stress on the adrenal cortex of rats. Material and methods: Thirty-five rats were distributed into four equally groups (control, sham control, heat stress either 30 minutes (min) or 60 min, vitamin E+ heat stress either 30min or 60min). Serological assessment of adrenocorticotropic hormone (ACTH), corticosterone, aldosterone and interleukin 1β were measured after the end of the experiment, in addition to measurements of malondialdehyde (MDA) in the tissue, superoxide dismutase (SOD) as well as gene expression of both CYP11B1 and CYP11B2 genes. Using haematoxylin and eosin (H&E) staining, specimens of suprarenal glands were examined histologically. Following immunohistochemical labelling for both Ki67 and caspase-3, histomorphometric analysis was performed. Results: Rats exposed to heat stress showed significantly higher serum levels of ACTH, corticosterone, aldosterone, MDA, and interleukin 1β, while SOD levels were decreased and CYP11B1 and CYP11B2 genes were upregulated. A significant increase in heat stress 60min versus, heat stress 30min and vitamin E treated groups. Light microscopic examination of the heat stressed rats revealed disorganized three zones of the suprarenal cortex, shrunken cells, vacuolations and congestion. Strong Caspase-3 immune-reactivity and abundant positive Ki67 nuclei were observed. The previous mentioned results were more pronounced in heat stress 60 min versus heat stress 30 min. Prophylactic administration of vitamin E prevented the previously mentioned alterations. Conclusion: Heat stress induced biochemical, histological and histomorphometric alterations in the suprarenal gland of rats, which is more pronounced in heat stress 60 min versus heat stress 30 min. Prophylactic administration of vitamin E ameliorated these harmful effects, which might be possibly due to its antioxidant property. Keywords: suprarenal gland, cortex, heat stress, vitamin E, antioxidant. | ||
| Keywords | ||
| Keywords: suprarenal gland; cortex; heat stress; vitamin E; antioxidant | ||
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