Characterization of extended-spectrum beta-lactamases produced by Enterobacteriaceae other than Escherichia coli and Klebsiella pneumoniae isolated in University Hospital of Monastir-Tunisia | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 02 December 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.327730.2280 | ||||
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| Authors | ||||
Yosr Kadri; Ons Haddad; Yassmine Maatouk   ; Mouna Ben Nejma; Maha Mastouri
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| Department of Microbiology, Fattouma-Bourguiba University Hospital, Monastir, University of Monastir, 5000 Monastir, Tunisia | ||||
| Abstract | ||||
| Background: The aims of this study were to determine the prevalence of Extended-spectrum-beta-lactamases (ESBL)-producing Enterobacteriaceae other than Escherichia coli and Klebsiella pneumoniae, to evaluate antimicrobial susceptibility patterns, and to characterize the ESBL genes involved in this resistance. Methods: This was a descriptive study carried out from December 2018 to November 2019. A total of 580 isolates were collected from Microbiology Laboratory of the University Hospital of Monastir, Tunisia. Antibiotic susceptibility to a panel of antibiotics was performed by the disk diffusion according to CA-SFM recommendations and ESBL production was detected by the double disk synergy test and E-test methods. Genes encoding ESBL (blaTEM, blaCTM, blaSHV) and blaCTX-M subgroups were identified by PCR. Results: Sixty-eight ESBL-producing Enterobacteriaceae other than E. coli and K. pneumoniae were identified among the 580 isolates, 85.3% of which were positive for at least one of the three genes (blaTEM, blaCTX-M, blaSHV), with blaTEM accounting for 73.5%, followed by blaCTX-M (52.9%). The bacterial strains were isolated mainly from pus in nosocomial infections. blaTEM was detected in all Proteus mirabilis isolates and in 85.0% of Enterobacter cloacae. blaCTX-M was frequently identified in E. cloacae and blaCTX-M-15 was the predominant subgroup among all CTX-M-1 positive isolates. About 30.9% ESBL-producing isolates harbored blaTEM + blaCTX-M combination with the predominant genotype found in 45.0% of E. cloacae. Conclusion: We report the increasing prevalence of ESBL-producing Enterobacteriaceae other than E. coli and K. pneumoniae, mainly due to the dissemination of blaTEM and blaCTX-M genes. ESBL-producing strains showed resistance to antibiotic classes other than beta-lactams. | ||||
| Keywords | ||||
| Resistance; Extend-spectrum-beta-lactamases; blaTEM; blaCTX-M; blaSHV | ||||
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