Biosynthesized Selenium Nanoparticles from Micropropagated Plectranthus Amboinicus Extract Revealing Antibacterial Activity
Mahmoud, H., Zaky, A., Elshenawy, H., Heikal, A. (2025). Biosynthesized Selenium Nanoparticles from Micropropagated Plectranthus Amboinicus Extract Revealing Antibacterial Activity. EKB Journal Management System, 68(7), 633-641. doi: 10.21608/ejchem.2024.333676.10735
Hatem Kamal Mahmoud; Ahmed Abbas Zaky; Hanaa Mohamed Elshenawy; Alaa Moustafa Heikal. "Biosynthesized Selenium Nanoparticles from Micropropagated Plectranthus Amboinicus Extract Revealing Antibacterial Activity". EKB Journal Management System, 68, 7, 2025, 633-641. doi: 10.21608/ejchem.2024.333676.10735
Mahmoud, H., Zaky, A., Elshenawy, H., Heikal, A. (2025). 'Biosynthesized Selenium Nanoparticles from Micropropagated Plectranthus Amboinicus Extract Revealing Antibacterial Activity', EKB Journal Management System, 68(7), pp. 633-641. doi: 10.21608/ejchem.2024.333676.10735
Mahmoud, H., Zaky, A., Elshenawy, H., Heikal, A. Biosynthesized Selenium Nanoparticles from Micropropagated Plectranthus Amboinicus Extract Revealing Antibacterial Activity. EKB Journal Management System, 2025; 68(7): 633-641. doi: 10.21608/ejchem.2024.333676.10735

Biosynthesized Selenium Nanoparticles from Micropropagated Plectranthus Amboinicus Extract Revealing Antibacterial Activity

Egyptian Journal of Chemistry
Volume 68, Issue 7, July 2025, Page 633-641  XML PDF (2.04 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.333676.10735
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Authors
Hatem Kamal Mahmoud1; Ahmed Abbas Zaky2; Hanaa Mohamed Elshenawy1; Alaa Moustafa Heikal email 1
1National Research Centre
2National Institute of Laser Enhanced Sciences
Abstract
Green synthesis of selenium nanoparticles using an aqueous extract of in vitro-derived Plectranthus amboinicus leaves has been biosynthesized. To produce in-vitro-derived plants of P. amboinicus to act as an efficient reductive solution for biosynthesizing SeNPs, a new tissue culture protocol was conducted using Spirulina plantensis extract as a natural growth regulator and secondary metabolites elicitor compatible with the adequate concentration of carbon source. Reaching the highest shoot length at 9.0 ± 0.42 and the maximum number of leaves per shoot at 15.0 ± 0.57, the enhancement of total phenolic contents revealed the highest level at 96.4 mg/g on MS supplemented with 1.0 mg of S. plantensis extract and 20 gm of sucrose. The enhancement of total phenolic compounds resulted in biosynthesized SeNPs with spherical-like shape and an average diameter of 17.9 ± 1.34 which was verified with Edx, SEM, TEM, and FTIR analyses. The biosynthesized SeNPs revealed antibacterial activity against the oral pathogens Escherichia coli and Staphylococcus aureus indicating the potential of biosynthesized selenium nanoparticles to be future alternatives to antibiotics losing their activity over time and to be combined with laser applications to intensify their activity in enchanting material delivery to targeted tissues and organs
Keywords
Biosynthesized SeNPs; Plectranthus Amboinicus; Spirulina plantensis (SE)
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