Isolation, Identification, and Antibiotic Resistance of Salmonella Spp. Strains Isolated From Broilers and Layer Hens In The Central Region of Algeria | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 05 December 2024 PDF (1.24 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2024.321538.2379 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sihem Guechtouli1; Chafik Redha Messai2; Amirouche Morsli  3; Taha Mossadak Hamdi4; Djamel Khelef5
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| 1Departement of Agricultural Sciences, Faculty of Sciences, M’Hamed Bougara University, Boumerdes, Algeria Laboratory of Food Hygiene and Quality Insurance System (HASAQ), High National Veterinary School, Algiers, Algeria | ||||
| 2Department of Biology and Agronomy, University Mohamed El Bachir El Ibrahimi, Bordj Bou Arreridj, Algeria Laboratory of Food Hygiene and Quality Insurance System (HASAQ), High National Veterinary School, Algiers, Algeria | ||||
| 3Faculty of science, Department of Agronomy, University M’Hamed Bougara of Boumerdes, Algeria. Laboratory of Bioinformatics, Applied Microbiology and Biomolecules (BAMB). M’Hamed Bougara university, Boumerdes, Algeria. | ||||
| 4Laboratory of Food Hygiene and Quality Insurance System (HASAQ), High National Veterinary School, Algiers, Algeria | ||||
| 5Laboratory of Health and Animal Production (SPA), High National Veterinary School, Algiers, Algeria | ||||
| Abstract | ||||
| The aim of this work is to isolate strains of Salmonella spp from broiler chickens and layer hens to identify their serotypes and to study their resistance profile.A total of 270 samples including liver and spleen were collected from 26 broiler farms and 25 layer hen farms. These samples include 140 broiler samples and 130 layer hen samples collected from different poultry farms in central Algeria. Strains of Salmonella spp were isolated and identified according to their macroscopic, microscopic and biochemical characteristics. The identification of the isolated strains was followed by serotyping of the isolates. Using Mueller-Hinton agar and the disk diffusion method, the resistance of the isolates to twelve antibiotic compounds was investigated. Among the 270 samples, 80 were positive (29.63%). In broiler chickens, 34 (24.28%) samples were confirmed positive, while in layer hens 46 (35.38%) samples were confirmed positive. Two serotypes from the 80 strains of Salmonella were identified with a predominance of the Salmonella Gallinarum serotype with a prevalence of 100% in layer hens and 79% in broiler chickens, followed by the Salmonella Enteritidis serotype. The highest resistances were recorded for Nalidixic acid NA (79.41%/100%), enrofloxacin ENR (41.18%/73.91%) and tetracycline Tcy (8.82%/54.35%), respectively in broiler chickens and the layer hen. However, no resistance to colistin was recorded in either species.48 isolates (60%) were multi drug resistant (MDR), and 3 antibiotic resistance profiles were recorded, with a multi-antibiotic resistance index (MARI) of 0.2 to 0.6.These findings demonstrated the risk that chicken farming poses to the persistence and spread of salmonella strains that are resistant to several antibiotics. | ||||
| Keywords | ||||
| Salmonella; broiler chicken; laying hen; multidrug resistance; serotype | ||||
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