The Possible Therapeutic Effect of Mesalazine Enema Versus Platelet Rich Plasma on Experimentally Induced Inflammatory Bowel Disease: Biochemical, Histological, and Morphometric Studies. | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 05 December 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2024.327906.2158 | ||||
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| Authors | ||||
Amal Ahmed Farag 1; Abeer Ibraheem Omar 2; Asmaa El-Shafei  1
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| 1Department of Histology, Faculty of Medicine, Cairo University, Egypt | ||||
| 2Histology department, Faculty of Medicine, Cairo University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Inflammatory bowel disease (IBD) is a chronic condition characterized by inflammation of the gastrointestinal tract, including colitis. Mesalazine enema and platelet-rich plasma (PRP) have shown promise efficacy in treating IBD. This study aimed to compare the effects of mesalazine enema and PRP on macroscopic parameters, histological findings, and biochemical markers in a rat model of induced colitis. Material and Methods: The study used a rat model of induced colitis. Rats were divided into control & experimental groups. The latter were subdivided into colitis, untreated recovery, mesalazine enema-treated, and PRP-treated subgroups. Macroscopic parameters were assessed using the Disease Activity Index (DAI) and macroscopic colonic scores. Histological evaluation included H&E & Masson's trichrome stain, PAS-AB reaction, and immunohistochemical staining for NF-kB and PCNA. Results: Both mesalazine enema and PRP treatment significantly reduced DAI and macroscopic colonic scores compared to the colitis and untreated recovery subgroups. However, the mesalazine enema-treated subgroup showed a significantly greater reduction in both parameters compared to the PRP-treated subgroup. Histological analysis revealed that the mesalazine enema-treated subgroup had nearly normal colonic wall architecture, intact surface epithelium, regular crypt arrangement, and minimal inflammatory cell infiltration. The PRP-treated subgroup showed signs of regeneration, including partial restoration of the surface epithelium and some regular crypts. The mesalazine enema-treated subgroup also exhibited a more prominent distribution of goblet cells compared to the PRP-treated subgroup. However, the PRP-treated subgroup had a higher number of cells with PCNA immunoreactivity compared to the mesalazine enema-treated subgroup. Conclusion: Both mesalazine enema and PRP treatment showed beneficial effects on macroscopic parameters, histological findings, and biochemical markers in the rat model of induced colitis. However, mesalazine enema demonstrated superior efficacy, resulting in a greater reduction in disease severity and improved histological parameters. These findings suggest that mesalazine enema may be more potent therapeutic option for managing colitis. | ||||
| Keywords | ||||
| Colitis; mesalazine enema; platelet-rich plasma; NF-kB; PCNA | ||||
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