The Antiangiogenic Activity of Anti-IL-6 and Anti-IL-8 in the Tumor Microenvironment of Breast Cancer (Preclinical Study)
Abou Shousha, S., Baheeg, S., Abo El-Wafa, A., Motawea, M., Sheta, M., Shahine, Y. (2025). The Antiangiogenic Activity of Anti-IL-6 and Anti-IL-8 in the Tumor Microenvironment of Breast Cancer (Preclinical Study). EKB Journal Management System, 2(1), 24-33. doi: 10.21608/japs.2024.287068.1025
Seham M. Abou Shousha; Suzan Baheeg; Ahmed Abo El-Wafa; Mohamed Atef Motawea; Manal Sheta; Yasmine M. Shahine. "The Antiangiogenic Activity of Anti-IL-6 and Anti-IL-8 in the Tumor Microenvironment of Breast Cancer (Preclinical Study)". EKB Journal Management System, 2, 1, 2025, 24-33. doi: 10.21608/japs.2024.287068.1025
Abou Shousha, S., Baheeg, S., Abo El-Wafa, A., Motawea, M., Sheta, M., Shahine, Y. (2025). 'The Antiangiogenic Activity of Anti-IL-6 and Anti-IL-8 in the Tumor Microenvironment of Breast Cancer (Preclinical Study)', EKB Journal Management System, 2(1), pp. 24-33. doi: 10.21608/japs.2024.287068.1025
Abou Shousha, S., Baheeg, S., Abo El-Wafa, A., Motawea, M., Sheta, M., Shahine, Y. The Antiangiogenic Activity of Anti-IL-6 and Anti-IL-8 in the Tumor Microenvironment of Breast Cancer (Preclinical Study). EKB Journal Management System, 2025; 2(1): 24-33. doi: 10.21608/japs.2024.287068.1025

The Antiangiogenic Activity of Anti-IL-6 and Anti-IL-8 in the Tumor Microenvironment of Breast Cancer (Preclinical Study)

Journal of Advanced Pharmaceutical Sciences
Volume 2, Issue 1, January 2025, Page 24-33  XML PDF (1.08 MB)
Document Type: Original Article
DOI: 10.21608/japs.2024.287068.1025
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Authors
Seham M. Abou Shousha1; Suzan Baheeg2; Ahmed Abo El-Wafa2; Mohamed Atef Motawea3; Manal Sheta3; Yasmine M. Shahine email 4
1Department of Immunology and Allergy, Medical Research Institute, Alexandria University, Egypt
2National Institute of Oceanography and Fisheries, Central Laboratories Unit, Alexandria Egypt.
3Department of Surgery, Medical Research Institute, Alexandria University, Alexandria, Egypt
4Microbiology & Immunology Department, Faculty of Pharmacy, Pharos university in Alexandria, Alexandria , Egypt
Abstract
The tumor microenvironment of breast cancer is considered a vibrant, multifactorial milieu that contains different cell subsets that shape the tumor fate. In addition, cytokines play a vital role within the tumor microenvironment, where different cytokines can promote inflammation, angiogenesis, and immune suppression. Interleukin-8 (IL-8) and Interleukin-6 (IL-6) are two of the most important cytokines that aid tumor progression and metastasis. Thus, this study aimed to compare the anti-angiogenic actions of neutralizing antibodies against IL-8 and IL-6. Tumor tissues from 30 patients having mastectomy supplemented with either monoclonal neutralizing antibodies against IL-8 or IL-6 were used to evaluate their antiangiogenic effect by evaluating (Vascular endothelial growth Factor) VEGF expression and microvessels count. It was demonstrated that Anti-IL-8 mAb declined significantly the number of microvessels in the tumor tissues compared to Anti-IL-6 and the control tissue cultures p<0.0001. In addition, the decrease in the microvessels count was insignificant within the untreated normal tissues (p=0.46). Therefore, the antiangiogenic activity of Anti-IL-8 monoclonal antibody is more potent than Anti-IL-6 according to the study conditions.

Highlights
  • Breast cancer tumor microenvironment is a promising target for cancer immunotherapy.
  • IL-6 and IL-8 have a vital role in angiogenesis promotion in breast cancer.
  • Anti-IL-8 monoclonal antibody has a superior effect in angiogenesis inhibition compared to Anti-IL-6.
Keywords
Angiogenesis; Interleukin 8; Interleukin 6; VEGF
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