Study the association between rs1800629 polymorphism in tumor necrosis factor alpha gene and insulin-dependent diabetes mellitus
Gouda, W., Mageed, L., Abd El Dayem, S., Afify, M. (2025). Study the association between rs1800629 polymorphism in tumor necrosis factor alpha gene and insulin-dependent diabetes mellitus. EKB Journal Management System, 68(8), 559-565. doi: 10.21608/ejchem.2024.328314.10626
Weaam Gouda; Lamiaa Mageed; Soha M Abd El Dayem; Mie Afify. "Study the association between rs1800629 polymorphism in tumor necrosis factor alpha gene and insulin-dependent diabetes mellitus". EKB Journal Management System, 68, 8, 2025, 559-565. doi: 10.21608/ejchem.2024.328314.10626
Gouda, W., Mageed, L., Abd El Dayem, S., Afify, M. (2025). 'Study the association between rs1800629 polymorphism in tumor necrosis factor alpha gene and insulin-dependent diabetes mellitus', EKB Journal Management System, 68(8), pp. 559-565. doi: 10.21608/ejchem.2024.328314.10626
Gouda, W., Mageed, L., Abd El Dayem, S., Afify, M. Study the association between rs1800629 polymorphism in tumor necrosis factor alpha gene and insulin-dependent diabetes mellitus. EKB Journal Management System, 2025; 68(8): 559-565. doi: 10.21608/ejchem.2024.328314.10626

Study the association between rs1800629 polymorphism in tumor necrosis factor alpha gene and insulin-dependent diabetes mellitus

Egyptian Journal of Chemistry
Volume 68, Issue 8, August 2025, Page 559-565  XML PDF (225.62 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.328314.10626
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Authors
Weaam Gouda email orcid 1; Lamiaa Mageedorcid 1; Soha M Abd El Dayemorcid 2; Mie Afifyorcid 1
1Biochemistry Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt
2Pediatrics Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Abstract
Background: Cytokines have vital roles in the autoimmune disease known as insulin-dependent diabetic mellitus (IDDM), which is genetically predisposed. Several studies elucidated that tumor necrosis factor-alpha gene (TNF-α) polymorphism was related to the evolution of diabetes mellitus. The current study was aimed to evaluate the cytokines TNF-α and interleukin-6 (IL-6) levels as well as to assess the gene polymorphism of TNF-α rs1800629 (-308G/A) variations among diabetic children.
Subjects and Methods: The study was carried out on 161 IDDM cases and 120 non-diabetic subjects. The cytokine TNF-α and IL-6 levels were evaluated in serum by enzyme-linked immunosorbent assay (ELISA). The TNF-α -308G/A (rs1800629) gene polymorphism was examined with the real-time polymerase chain reaction (RT-PCR) method.
Results: Serum levels of TNF-α and IL-6 were elevated among IDDM patients in comparison to controls, and a positive correlation was indicated between TNF-α and IL-6 (r = 0.235, p<0.001). The association analysis showed a significant difference in the TNF-α gene between patients and controls. Specifically, the patients with the A/A genotype had a considerably greater risk of IDDM compared with the control.
Conclusion: The findings indicated that the mutant TNF-α (308 A/A) (rs1800629) was a risk factor for IDDM vulnerability in children. Hence, TNF-α plays an essential role in the evolution and pathogenesis of IDDM, and it might be a useful indicator of the disease's progression.
Keywords
Insulin-dependent diabetes mellitus (IDDM); Tumor necrosis factor-alpha (TNF-α); Interleukin 6 (IL-6); Diabetes mellitus; Gene polymorphism
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