Antimicrobial Resistance Gene and Molecular Properties of Escherichia Coli Isolated from Meat Products in the El-Gharbia Governorate | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 09 December 2024 PDF (1.12 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.326845.2413 | ||
| Authors | ||
| Reda Mohammed Abdallah* 1; Eman Mohammed Abdelhady2; Nader Yehia Mostafa1; Ghada A.K. Kirrella1; Khaled A. Abd El-Razik3; Ehab Ali Fouad4 | ||
| 1Food Control Department, Faculty of Veterinary Medicine, Kafrelsheikh University Egypt. | ||
| 2Food Control Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt | ||
| 3Department of Animal Reproduction, Veterinary Research Institute, National Research Centre, Giza, Egypt | ||
| 4Department of Zoonosis, Veterinary Research Institute, National Research Centre, Giza, Egypt. | ||
| Abstract | ||
| the current investigation's objective was to identify Escherichia coli (E. coli), its virulence factors, antibiotic-resistant pattern(s), and antibiotic-resistant genes. A total of sixty samples were collected from diverse supermarkets and grocery stores located in El-Gharbia Governorate, consisting of 20 minced meat samples, 20 sausages, 10 burgers, and 10 luncheon meats. Through culturing and biochemical assays, 45 samples, representing 75%, tested positive for E. coli in the meat products. The prevalence was notably higher in minced meat and sausages, both at 100%, while burgers and luncheon meats showed lower rates of 40% and 0%, respectively. To evaluate the antimicrobial susceptibility of the E. coli samples, eleven different antimicrobial discs, which are among the most commonly used, were employed. Antibiotics including ceftriaxone, amoxicillin/clavulanic acid, ceftazidime, and erythromycin demonstrated notable sensitivity rates of 73.3%, 66.7%, and 64.4%, respectively. In contrast, there were considerable resistance levels observed for ciprofloxacin, azithromycin, and gentamicin, with resistance rates of 60%, 57.8%, and 51.5%, respectively. A PCR used to identify E. coli by looking for the phoA (alkaline phosphatase) gene showed that all samples tested positive for the bacteria. In 91.11%, 88.88%, 86.66%, and 24.44% of E. coli samples, the eaeA ( intimin), tsh (temperature-sensitive hemagglutinin), tetA (tetracycline), and mcr1(colistin) genes were discovered, respectively. The aggregate findings suggest that consuming tainted meat products may be a potential means of spreading pathogenic E. coli. Concerns have been raised over the potential function of meat products as a reservoir for pathogenic E. coli due to the detection of the tsh gene mostly or solely in Avian Pathogenic E. coli (APEC). When combined, this may present a therapeutic problem or challenges for the consumption of meat products as well as a zoonotic risk to humans. | ||
| Keywords | ||
| E. coli; Meat products; phoA; eaeA; tsh; tetA; mcr1; PCR | ||
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