Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type
Alquraishi, M., Al-Bayati, M., AlFatlawi, M., Alamer, N., Alzeyadi, M. (2024). Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type. EKB Journal Management System, 10(5), 169-179. doi: 10.21608/jbaar.2024.396492
Mohammed K. S. Alquraishi; Maha Mashrq Al-Bayati; Mustafa Hamza AlFatlawi; Noor Alamer; Mohammad Alzeyadi. "Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type". EKB Journal Management System, 10, 5, 2024, 169-179. doi: 10.21608/jbaar.2024.396492
Alquraishi, M., Al-Bayati, M., AlFatlawi, M., Alamer, N., Alzeyadi, M. (2024). 'Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type', EKB Journal Management System, 10(5), pp. 169-179. doi: 10.21608/jbaar.2024.396492
Alquraishi, M., Al-Bayati, M., AlFatlawi, M., Alamer, N., Alzeyadi, M. Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type. EKB Journal Management System, 2024; 10(5): 169-179. doi: 10.21608/jbaar.2024.396492

Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type

Journal of Bioscience and Applied Research
Volume 10, Issue 5, December 2024, Page 169-179  XML PDF (1.58 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2024.396492
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Authors
Mohammed K. S. Alquraishi* 1; Maha Mashrq Al-Bayati2; Mustafa Hamza AlFatlawi1; Noor Alamer1; Mohammad Alzeyadi3
1Department of Pathological Analyses, Faculty of Science, University of Kufa, Iraq
2Department of Medical Laboratory Technology, Faculty of Medical Technology, University of Imam Jaafar Sadiq, Najaf, Iraq
3Department of Biology, Faculty of Science, University of Kufa, Iraq
Abstract
Using intelligent computer programs, the secondary structure and thermodynamic properties of hsa-mir-181a ACE2 were investigated in both vaccinated and unvaccinated samples, comparing them to the wild type. By utilizing the RNAalifold web server, a consensus structure of aligned micro-RNA sequences was predicted, along with the minimum free energy (MFE) and equilibrium base-pairing probabilities. The results showed a strong correlation between the secondary structure of hsa-mir-181a ACE2 and the vaccination status, with a higher MFE observed in the vaccinated group. This study proved a significant relationship between the secondary structure of hsa-mir-181a ACE2 and the selected class of vaccinated or unvaccinated. The value of (MFE) of the secondary structure of microRNA increased in vaccinated compared with unvaccinated, with a significant difference evident. Also, at the thermodynamic free energy (value) level, the vaccination was increased compared to the unvaccinated as the difference was clear and the relationship was achieved.
Keywords
MicroRNA; Computational microRNA; Minimum free energy; Thermodynamic free energy; The RNAalifold web server
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