Method Optimization and Validation of Antibiotics Residues in Milk Sample Using LC-MS/MS | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Article 20, Volume 56, Issue 8, August 2025, Page 1867-1878 PDF (694.22 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2024.328832.2429 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Jagdish Kumar Parmar1; Shoorvir Singh2; Vikas Gupta3; Umesh Kumar  4
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| 1Department of Biotechnology, GLA University, Mathura 281406, Uttar Pradesh, India | ||||
| 2Department of Biotechnology, GLA University, Mathura 281406, U.P, INDIA | ||||
| 3IRCLASS System and Solutions Pvt Ltd, Jaipur 302017, Rajasthan, India | ||||
| 4Department of Biosciences, IMS Ghaziabad University Courses Campus, NH09, Ghaziabad , Uttar Pradesh 201015 India | ||||
| Abstract | ||||
| Veterinary drugs, that are utilized to treat diseases in animals, are broad-spectrum antibacterial antibiotics. Numerous nations have previously prohibited the utilization of veterinary medications due to the formation of residues for example nitrofurans, chloramphenicol etc. Veterinary drugs have already been supplied to animals to enhance milk production for increased profit and economic incentives. The rising utilization of illegal medicines in animal milk production is largely ineffective for consumers. It affects food products derived from animals when consumers purposefully consume the medication since it leaves behind many antimicrobial residues. It causes serious health problems in milk consumers, including allergic disorders as well as cancer. This necessitates a limitation on illegal consumption of drugs for veterinary purposes; the Indian government needs to put restrictions on this drug utilization in animals. It is possible to find and confirm the presence of different veterinary medication residues in milk; dairy products can be examined with liquid chromatography and mass spectrometry, among other analytical methods. These are all advanced devices, and the requisite equipment is now readily accessible. According to the European Commission, 2021/808/EC should govern the validation of analytical methods. The application of veterinary drugs consistently presents significant challenges regarding efficiency, prolonged usage, and authorization. Various analytical procedures can be efficiently employed to protect consumer health in a short time; multiple compound groups, including tetracyclines, macrolides, sulphonamides etc. are analyzed utilizing a single multi-residual technique. Each of these techniques is employed within predetermined validation parameters, which consist of precision, quantification, accuracy, detection limit, along with calibration curve. This current research aims to develop a multi-residual fast test for milk samples in a reduced timeframe. | ||||
| Keywords | ||||
| Veterinary Drugs Residues; LC-MS/MS; Method Validation; Method development; Antibacterial | ||||
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