INFLUENCE OF DEAE-DEXTRAN ON THE YIELD OF PARAINFLUENZA-3 (PI-3) VIRUS PROPAGATED ON DIFFERENT CELL CULTURE SYSTEMS | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 28, Volume 79, Issue 1, March 2001, Page 361-372 PDF (4.34 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2001.397699 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| SAMIRA S. TAHA | ||||
| Veterinary Serum and Vaccine Research institute, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| Infectivity titre and haemagglutination titre of PI-3 virus in MDBK cells using monolayer, suspension and roller systems revealed that the roller system produced the highest infectivity and haemagglutination in-hibition titre with an increase of loge 08 than their corresponding mono-layer cell culture. On the other hand, the infectivity and HA titres in cell suspension cultures were 1.0 logs 0 lower than their corresponding mon-olayer cell culture system. Influence of DEAE-Dextran for the enhancement of P1-3 virus in-fectivity in different cell culture systems, 25ug of DEAE-Dextran per ml is considered the safest concentration to the MDBK and VERO cell lines and giving these results: 1.Infectivity titre of PI-3 virus was markedly increased under the effect of both serial passages, and showed an increase of 1.5 logs 0 and 1.3 loge 0, respectively, more than their respective non-treated passages. 2.In the roller system, DEAE-Dextran enhanced the growth of virus by about 1.0 logi 0 and 1.2 logi 0 more than growth of untreated virus in both MDBK and VERO cell lines, respectively. 3.The highest titre obtained when using DEAE-Dextran MDBK roller sys-tem was 9.5 logs o. | ||||
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