The Role of Colchicine in the Prevention of Bleomycin-Induced Pulmonary Fibrosis in Rats | ||
| Egyptian Journal of Histology | ||
| Article 19, Volume 48, Issue 1, March 2025, Pages 293-305 PDF (6.16 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.340347.2182 | ||
| Authors | ||
| Sarah A. Arakib* 1; Mohammed Abdel Hay Autifi2; Eman A. Elsawaf3; Alsayed A. Abdelhady4; Manal H. AL Badawi5 | ||
| 1Anatomy and Embryology Department, Faculty of medicine, Helwan University | ||
| 2Head of Anatomy & Embryology Department Faculty of Medicine Al-azhar University Cairo, Egypt | ||
| 3Department of Anatomy and Embryology, Faculty of Medicine, Helwan University, Helwan University. | ||
| 4Anatomy and Embryology Department, Faculty of Medicine, Helwan University | ||
| 5Anatomy and embryology department, faculty of Medicine, Helwan University | ||
| Abstract | ||
| Introduction: Lung fibrosis is the most popular and serious sequel of interstitial inflammatory diseases and may lead to respiratory failure in critical cases with a mortality rate of 10% to 20%. Its etiology includes chemicals, infections, radiation, allergens, drugs such as bleomycin (BLM), and as a result of the coronavirus disease 2019 (COVID-19). Aim of the Work: The purpose of the present work was to detect the preventive outcome of colchicine on lung fibrosis in rats. Material and Methods: 24 male albino rats were subdivided into 4 groups, 6 rats per group; group I (control G), group II (colchicine G), group III (BLM G), and group IV (BLM+colchicine G). The lung fibrosis was done by injection of BLM intraperitoneally (dose; 0.5 mg\kg 2 times \ week for 3 weeks). BLM+colchicine group was taken BLM as in group III in addition colchicine was taken daily (dose; 1 mg/kg by oral gavage for 3 weeks). By the study period end (3 weeks), the rats were put under anesthesia and the thoracic wall was opened. The lungs were taken and prepared for H&E, Masson’s trichrome, and immunohistochemical staining. Some specimens were prepared and examined by transmission electron microscope (TEM). Results: The histological changes in the lungs of the BLM group were deformed pulmonary architecture, thickened interstitial walls, alveolar collapse, severe invasion by inflammatory cells, highly significant collagen fibrils deposition, severe positive reaction in the interstitial walls by anti-α-SMA, and degenerated pneumocytes I and II by TEM. The preventive group showed restoration of most of the normal lung architecture and a marked decrease in inflammatory and fibrotic changes. Conclusion: Colchicine can lessen the histopathological and ultrastructural changes in the lungs resulting from BLM. | ||
| Keywords | ||
| α-SMA; bleomycin; clchicine; pulmonary fibrosis; rats | ||
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