Expression and Subcellular Distribution of Membrane-Organizing Extension Spike Protein (Moesin/ MSN) are Associated with Epithelial-Mesenchymal Transition in Clear Cell Renal Cell Carcinoma | ||||
| Sohag Medical Journal | ||||
| Volume 29, Issue 1, 2025, Page 54-65 PDF (1.69 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/smj.2024.337520.1513 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Noha ED Hassab El-Naby; Nagwa Abd El-Sadek Ahmed; Maisa Hashem Mohammed  
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| Pathology Department, Faculty of Medicine, Sohag University, Sohag, Egypt | ||||
| Abstract | ||||
| Background: Clear cell renal cell carcinoma (ccRCC) is an aggressive urological malignancy. The aggressive potential of ccRCC is attributed to epithelial-mesechymal transition, which acquire the neoplastic cells more invasive properties. Membrane-Organizing Extension Spike Protein (Moesin/ MSN) plays an important role in controlling cellular morphology and motility. Aim: Evaluation of moesin expression in ccRCC and determine its cellular distribution, and then correlate its expression with certain clinicopathological variables. Methods: Archived formalin-fixed, paraffin-embedded ccRCC tissue blocks of 55 patients were obtained and sectioned. From each tissue block; 2 tissue sections were stained by hematoxylin and eosin (H&E) stain and anti-moesin immunohistochemical antibody. Results: Less differentiated cases of ccRCC with high WHO/ISUP grades were significantly associated with male sex (p= 0.023), presence of perirenal fat invasion (p< 0.001) and advanced tumor stages (p= 0.003). Moesin showed membranous localization in all enrolled cases. Membranous moesin expression was associated with high WHO/ISUP grades (p= 0.004), advanced tumor stages (p< 0.001), capsular and perirenal fat invasion (p= 0.001& p< 0.001) and presence of lymph nodes invasion (p< 0.001). Cytoplasmic redistribution of moesin was detected in 30 cases. Cytoplasmic moesin was correlated with capsular and perirenal fat invasion (p= 0.033& p= 0.001), tumors with high WHO/ISUP grades (p< 0.001), advanced tumor stages (p< 0.001) and presence of nodal metastasis (p= 0.002). Conclusion: Membranous overexpression of moesin and its cytoplasmic redistribution were detected in aggressive and less differentiated cases of clear cell renal cell carcinoma. | ||||
| Keywords | ||||
| Renal Cell Carcinoma; Moesin; Epithelial-Mesenchymal Transition; Immunohistochemistry | ||||
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