ANTIMICROBIAL ACTIVITY OF POLYKETIDE-DERIVED SECONDARY METABOLITES FROM DOTHIDEOMYCETES SP. | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Volume 48, Issue 1, June 2025, Pages 229-240 PDF (1.35 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2024.327771.2312 | ||
| Authors | ||
| lourin Gamal Malak* 1; mohamed Ibrahim2; Daneel Ferreira3; John Williamson4; Elizabeth Arnold5; mohamed Abouelela6; Samir Ross7 | ||
| 1Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut, Egypt, 71526 National Center for Natural Products Research University, Mississippi 38677-1848, United States | ||
| 2National Center for Natural Products Research, University, Mississippi 38677-1848, United States | ||
| 3Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University, Mississippi 38677-1848, United States | ||
| 4Institute of Cannabis Research, Colorado State University-Pueblo, Pueblo, CO 81001, United States | ||
| 5School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, United States | ||
| 6Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut-Branch, Assiut, 71524, Egypt | ||
| 7National Center for Natural Products Research, Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University, Mississippi 38677-1848, United States | ||
| Abstract | ||
| Discovering alternative compounds for treating antibiotic-resistant infections is critical to alleviate the current antibiotic resistance crisis. Endophytic fungi are a source of numerous recently approved antibiotics particularly polyketide-derived molecules. This study aimed to isolate and identify antimicrobial compounds from Dothideomycetes sp. 11144 as well as molecular docking study for their affinity to different Methicillin-resistant Staphylococcus aureus (MRSA) selected proteins. Herein, we describe the isolation of four polyketide-type metabolites, a new natural α-pyrone derivative, (R)-6-(2-hydroxypropyl)-4-methoxy-2H-pyran-2-one (1) along with three known anthraquinone derivatives, chrysophanol (2), emodin (3), and coniothyrinone B (4) from Dothideomycetes sp. 11144 liquid culture. The extracts and isolated compounds were screened for their antimicrobial activity. (R)-6-(2-hydroxypropyl)-4-methoxy-2H-pyran-2-one (1) and emodin (3) exhibited antibacterial activity against MRSA with IC50 values of 61.0 and 24.4 µM, respectively. They were screened virtually via molecular docking for their affinity to different MRSA essential proteins compared to conventional inhibitors. Emodin demonstrated high affinity to the penicillin-binding protein with a pose score of -13.57 kcal/mol (1MWT). The emodin architecture could serve as a framework for the development of potent anti-MRSA antibiotics. | ||
| Keywords | ||
| antimicrobial; anti-MRSA; pyrone; Anthraquinones | ||
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