Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30
Abd-El Razik, M., El-Waseif, A., Abobaker, R., Emam, F., Hassan, M. (2024). Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30. EKB Journal Management System, 16(2), 177-187. doi: 10.21608/eajbsg.2024.399897
Mohamed Abd-El Razik; Amr A. El-Waseif; Rabea A. Abobaker; Ferial M. Emam; Mervat G. Hassan. "Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30". EKB Journal Management System, 16, 2, 2024, 177-187. doi: 10.21608/eajbsg.2024.399897
Abd-El Razik, M., El-Waseif, A., Abobaker, R., Emam, F., Hassan, M. (2024). 'Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30', EKB Journal Management System, 16(2), pp. 177-187. doi: 10.21608/eajbsg.2024.399897
Abd-El Razik, M., El-Waseif, A., Abobaker, R., Emam, F., Hassan, M. Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30. EKB Journal Management System, 2024; 16(2): 177-187. doi: 10.21608/eajbsg.2024.399897

Nano-Exopolysaccharide from Probiotic Lactobacillus brevis Impact against Cancer Cells Caco and HT-29 Development based on ROS, IL-6, IL-8, TNF-β and M30

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Volume 16, Issue 2, December 2024, Pages 177-187    PDF (692.11 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2024.399897
Authors
Mohamed Abd-El Razik1; Amr A. El-Waseif2; Rabea A. Abobaker1; Ferial M. Emam1; Mervat G. Hassan3
1Botany and Microbiology Dept., Faculty of Science, Suez Canal University, Egypt.
2Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
3Botany and Microbiology Dept., Faculty of Science, Banha University, Egypt.
Abstract
Probiotic developments recently have provided new avenues for the fight against cancer. Via making use of Lactobacillus brevis extract's reducing properties. Therefore, the current study investigated whether nano-exopolysaccharide (NEPS) from probiotic can decrease cell proliferation, increase apoptosis, and activate autophagy in CaCo2 and HT-29 cells using in vitro techniques. On the other hand, compared to non-apoptotic cells, apoptotic cells showed orange-colored bodies as a result of cellular shrinkage and hemorrhage brought on by NEPS stimulation. Not to be overlooked is the NEPS 's exceptional selectivity in causing cell death, particularly in CaCo2 and HT-29 cells. For CaCo2 and HT-29 cells, the half-maximum inhibitory concentration (IC50) values were 9.4 μg/ml and 6.2 μg/ml, respectively. In HT-29 cells, probiotics cause an increase in the synthesis of cytokines linked to inflammation, such as TNF-β, IL-8, and IL-6. As an outcome of apoptosis being triggered and immune system responses being strengthened, our study's findings suggest that using probiotics could be a practical approach to eliminating colorectal malignant cells.
Keywords
Probiotic; Lactobacillus brevis; Apoptosis; colon cancer; cytokines; nano-exopolysaccharide (NEPS)
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