Evaluation of cold-adapted lipolytic activity of some Cladosporium, Penicillium, and Talaromyces species
Omar, A., Zohri, A., Abdel-Malek, A., Al-Bedak, O. (2025). Evaluation of cold-adapted lipolytic activity of some Cladosporium, Penicillium, and Talaromyces species. EKB Journal Management System, 54(1), 24-43. doi: 10.21608/aunj.2024.316072.1088
Asmaa M.A. Omar; Abdel-Naser A. Zohri; Ahmed Y. Abdel-Malek; Osama A Al-Bedak. "Evaluation of cold-adapted lipolytic activity of some Cladosporium, Penicillium, and Talaromyces species". EKB Journal Management System, 54, 1, 2025, 24-43. doi: 10.21608/aunj.2024.316072.1088
Omar, A., Zohri, A., Abdel-Malek, A., Al-Bedak, O. (2025). 'Evaluation of cold-adapted lipolytic activity of some Cladosporium, Penicillium, and Talaromyces species', EKB Journal Management System, 54(1), pp. 24-43. doi: 10.21608/aunj.2024.316072.1088
Omar, A., Zohri, A., Abdel-Malek, A., Al-Bedak, O. Evaluation of cold-adapted lipolytic activity of some Cladosporium, Penicillium, and Talaromyces species. EKB Journal Management System, 2025; 54(1): 24-43. doi: 10.21608/aunj.2024.316072.1088

Evaluation of cold-adapted lipolytic activity of some Cladosporium, Penicillium, and Talaromyces species

Assiut University Journal of Multidisciplinary Scientific Research
Volume 54, Issue 1, January 2025, Page 24-43  XML PDF (732.61 K)
Document Type: Novel Research Articles
DOI: 10.21608/aunj.2024.316072.1088
View on SCiNiTO View on SCiNiTO
Authors
Asmaa M.A. Omarorcid 1; Abdel-Naser A. Zohriorcid 1; Ahmed Y. Abdel-Malekorcid 1; Osama A Al-Bedak email orcid 2
1Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt
2Assiut University Mycological Centre
Abstract
In this study, 216 isolates—104 Cladosporium, 94 Penicillium, and 18 Talaromyces—were evaluated for lipolytic activity at 10 °C on agar medium. Of the total isolates, 167 (77 Cladosporium, 81 Penicillium, and 9 Talaromyces) yielded positive results, accounting for 77.31 % of total. Of these, 40 isolates (14 Cladosporium, 25 Penicillium, and 1 Talaromyces) demonstrated high lipase activity, 54 moderate (25 Cladosporium, 22 Penicillium, and 7 Talaromyces), and 73 isolates (38 Cladosporium, 34 Penicillium, and 1 Talaromyces) displayed low lipolytic activity. All forty of the isolates from the first screening that had significant lipase activity at 10 °C were selected for the secondary screening under submerged fermentation conditions (SmF). Although in varying amounts, every isolate tested in the submerged fermentation secondary screening experiment was able to produce lipase. At 5 ºC, lipase's specific activity ranged from 6.1 to 353.4 U/mg protein; at 10 ºC, it was 3.83 to 353.34 U/mg; at 15 ºC, it was 6.77 to 463.75 U/mg; and at 20 ºC, it was 4.38 to 485.84 U/mg. Based on sequencing of internal transcribed spacer region (ITS), the two most active strains were identified as Cladosporium cladosporioides AUMC 15908 and Talaromyces pinophilus AUMC 308.
Keywords
Cladosporium; Lipase; Penicillium; Screening; Talaromyces
Statistics
Article View: 180
PDF Download: 243