Aspects of the Potential Artificial Spawning and Reproductive Performance of Solea aegyptiaca | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 117, Volume 28, Issue 6, November 2024, Page 2175-2193 PDF (487.08 K) | ||||
| DOI: 10.21608/ejabf.2024.400584 | ||||
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| Authors | ||||
| Safaa Aljilaney; Esam Aiaad | ||||
| Abstract | ||||
| The present experiments focused on induced spawning in Solea aegyptiaca using different hormones at varying doses compared to non-injected fish. Broodstock were collected from the 3rd depression of Wadi El-Rayan from December 2022 to March 2023. The experiments were divided into three groups. The first group of fish was intramuscularly injected with 4000, 5000, and 5200 IU HCG per kg of body weight, split into three trials. The second group was injected with CPH at 3.5, 4.0, and 4.5mg/ kg BW. The third group served as the control, receiving 0.9% saline water injections. This study compared the effectiveness of hormones on egg diameter, ovulation, fertilization, hatchability rate, and larval production. A total of 108 sole fish, with females weighing 55 ± 5g and males weighing 90.33 ± 1.25g, were used. Prior to primary injection, the oocytes were in the ripening period, with an average diameter of 600 ± 50µm. After hormone injections, egg diameters increased to a range of 927 ± 5.7 to 933 ± 12.2µm. The study showed that the longest spawning duration was 70 days for 28 batches of fish injected with 5000 IU HCG, while the shortest duration was 42 days for 8 batches of non-injected fish. The highest total number of fertilized eggs was 94,122 from fish injected with 5000 IU HCG, while the highest value from the CPH-treated fish was 30,506 at 4.0 mg CPH. The highest hatchability rate, 89%, was observed in fish injected with 5000 IU HCG, and the survival rate of metamorphosed larvae reached 71% at 30 days post-hatch (dph), decreasing to 25% at 60 dph. A significant difference (P< 0.05) was found during larval rearing. Based on these results, the best doses for induced spawning were determined to be 5000 IU of HCG and 4.0mg of CPH per kg of body weight. | ||||
| Keywords | ||||
| Solea aegyptiaca; Induced spawning; Human chorionic gonadotropin; Carp pituitary homogenate; Larval production | ||||
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