Improvement of inulin production in Jerusalem artichoke (Helianthus tuberosus L.) through foliar application of certain sugars
Sakr, D., Abdelsattar, M., Hathout, T., El khallal, S., Hassanein, S., Abdelgawad, Z. (2024). Improvement of inulin production in Jerusalem artichoke (Helianthus tuberosus L.) through foliar application of certain sugars. EKB Journal Management System, 41(2), 18-46. doi: 10.21608/jsrs.2024.265530.1124
Dina Ehab Sakr; Mohamed Abdelsattar; Tahani Abbas Hathout; Samia Moheb El khallal; Sameh Ebrahem Hassanein; Zeinab Ahmed Abdelgawad. "Improvement of inulin production in Jerusalem artichoke (Helianthus tuberosus L.) through foliar application of certain sugars". EKB Journal Management System, 41, 2, 2024, 18-46. doi: 10.21608/jsrs.2024.265530.1124
Sakr, D., Abdelsattar, M., Hathout, T., El khallal, S., Hassanein, S., Abdelgawad, Z. (2024). 'Improvement of inulin production in Jerusalem artichoke (Helianthus tuberosus L.) through foliar application of certain sugars', EKB Journal Management System, 41(2), pp. 18-46. doi: 10.21608/jsrs.2024.265530.1124
Sakr, D., Abdelsattar, M., Hathout, T., El khallal, S., Hassanein, S., Abdelgawad, Z. Improvement of inulin production in Jerusalem artichoke (Helianthus tuberosus L.) through foliar application of certain sugars. EKB Journal Management System, 2024; 41(2): 18-46. doi: 10.21608/jsrs.2024.265530.1124

Improvement of inulin production in Jerusalem artichoke (Helianthus tuberosus L.) through foliar application of certain sugars

Journal of Scientific Research in Science
Volume 41, Issue 2, 2024, Page 18-46  XML PDF (1.56 MB)
Document Type: Original Article
DOI: 10.21608/jsrs.2024.265530.1124
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Authors
Dina Ehab Sakr email orcid 1; Mohamed Abdelsattar2; Tahani Abbas Hathoutorcid 1; Samia Moheb El khallalorcid 1; Sameh Ebrahem Hassanein3; Zeinab Ahmed Abdelgawad1
1Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
2Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt
3School of Biotechnology, Nile University, Giza, Egypt.
Abstract
A field experiment was conducted at the experimental farm of the Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Centre (ARC) to investigate the response of Jerusalem artichoke plant to foliar application of Raffinose, Dextrin, and Sucrose at 10, 25, 50, and 100 mM in addition to control (tap water only) using Randomized complete block design with 3 replicates. Tested parameters were: plant fresh weight, dry weight, plant height, number of main branches/plants, number of lateral branches/plants, leaf area, photosynthetic pigment, total carbohydrate, inulin content, Fructan exohydrolase (FEH) activity, SDS-PAGE analysis of protein, soluble sugar content by HPLC, number of tubers produced, the yield per plant, and the overall yield per feddan (tons). Results indicated that spraying plants with Raffinose and Dextrin at 50 mM level enhanced all growth parameters, total carbohydrates, and inulin content. In addition, the dose of Raffinose 50 mM exhibits promising inhibition activity of FEH enzyme, increasing the production of inulin and tuber yield, followed by the same dose from Dextrin and Sucrose treatments. Based on this supposition, the results of this study suggested that the exogenous application of particular sugars, Raffinose and Dextrin, as an eco-friendly material, can improve the plant's overall growth, development, and yield of Jerusalem artichoke plant.
Keywords
Fructans; Fructan exohydrolase; Raffinose; Dextrin; Sucrose
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