Melatonin nanoparticles improve the growth and biochemical activities of Ocimum basilicum L. plant In vitro

Elshamy, Marwa Talat; Hathout, Tahani Abbas; El khallal, Samia Moheb; Fahmy, Ashraf Hussien; Hussein, Gihan M.; Gad, Rabab Abdulhai

doi:10.21608/jsrs.2024.280605.1126

	Melatonin nanoparticles improve the growth and biochemical activities of Ocimum basilicum L. plant In vitro
Journal of Scientific Research in Science
Volume 41, Issue 2, 2024, Page 71-94 PDF (991.23 K)
Document Type: Original Article
DOI: 10.21608/jsrs.2024.280605.1126
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Authors
Marwa Talat Elshamy ¹; Tahani Abbas Hathout ²; Samia Moheb El khallal ²; Ashraf Hussien Fahmy³; Gihan M. Hussein³; Rabab Abdulhai Gad²
¹Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
²Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt.
³Agriculture Genetic Engineering Research Institute (AGERI), Agricultural Research Center, Giza, Egypt.
Abstract
Melatonin (MEL) plays important roles in various plant physiology, particularly regulation of plant growth, development, and metabolism. The present study was carried out on Ocimum basilicum L. plant growing in a half-strength Murashige and Skoog media supplemented with 5, 10, 20, and 30 µM MEL-CS nanoparticles (NPs). After four weeks, growing O. basilicum plants were harvested for measuring the changes in growth, physiological, and biochemical attributes. Our results revealed that MEL-CS nanoparticles treatment markedly increased the shoot, and root’s length, fresh, and dry weight, photosynthetic pigments, carbohydrates fractions, total soluble proteins, free amino acids, and antioxidant enzymes activities (CAT, SOD, POD, and PPO). However, the accumulation of the superoxide radical (O₂•⁻), hydrogen peroxide (H₂O₂), as well as malonaldehyde (MDA) reduced in basil leaves. The most efficient treatment was 10 µM MEL-CS nanoparticles. At the same time, the concentration of 30 µM was the lowest effective compared to untreated control, normal MEL, and CS treatments. The positive results of current study proved the effective role of MEL-CS nanoparticles in improving plant growth, the measured metabolites, and thereby reducing the oxidative stress (O₂•⁻, H₂O₂, and MDA) through stimulating the antioxidant defense system.
Keywords
Ocimum basilicum L; Nanoparticles (NPs); Melatonin (MEL); Chitosan (CS)


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