Identification of multidrug-resistant Enterobacteriaceae harboring blaNDM-1gene isolated from surgical site infection at Benha University Hospital | ||||
Microbes and Infectious Diseases | ||||
Volume 6, Issue 2, May 2025, Page 701-709 PDF (683.04 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/mid.2024.340858.2376 | ||||
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Authors | ||||
Amira E. Ramadan ![]() | ||||
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt | ||||
2Department of general surgery, Faculty of Medicine, Benha University, Egypt | ||||
3Department of clinical and chemical pathology, Faculty of Medicine, Benha University, Egypt | ||||
4Department of medical biochemistry and molecular biology, Faculty of Medicine, Benha University, Egypt | ||||
Abstract | ||||
Background: Surgical site infection (SSI) is a major source of morbidity and mortality. It happens within 30 days of a surgical intervention around or at the incision site. Deeper underlying tissues may be involved. In recent years, multidrug-resistant (MDR) organisms are responsible for a growing proportion of illnesses, which present a significant therapeutic challenge. Aim: This study aimed to evaluate the antibiotic susceptibility pattern of Enterobacteriaceae isolated from SSI in Benha University Hospital and detect the blaNDM-1 gene in carbapenem resistant isolates. Methods: A total of 130 wound samples were cultured on blood agar and MacConkey agar media. Strains were identified by standard bacteriological methods and antibiotic sensitivity testing by VITEK 2 compact system, Detection of MBL (metallo-beta-lactamase) producing isolates phenotypically by double disk synergy test (DDST) and detection of blaNDM-1gene in the resistant strains by Conventional PCR. Results: of 120 culture positive wound samples, 53 strains were Enterobacteriaceae (22Klebsiella species, 20 E.coli, 8 Proteus spp and 3 Citrobacter spp), 59% and 30% of Klebsiella spp and E-coli strains respectively were carbapenem resistant, while none of Proteus or Citrobacter spp were carbapenem resistant. Regarding blaNDM-1 detection it was 61.54% and 66.67% for Klebsiella spp and E.coli respectively. Conclusion: NDM-1 gene propagation in Enterobacteriaceae is a worrying threat which necessitates several control measures. | ||||
Keywords | ||||
SSI; MDR; metallo-beta-lactamase; blaNDM-1 | ||||
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