Isolation and Identification of Bacteria Producing Indole From Rhizospheric Plant | ||
| Arab Universities Journal of Agricultural Sciences | ||
| Volume 32, Issue 2, December 2024, Pages 199-203 PDF (310.36 K) | ||
| Document Type: Short Communication | ||
| DOI: 10.21608/ajs.2024.305171.1577 | ||
| Authors | ||
| Dina M Saad* 1; Mohamed E El- Demerdash1; Yasmin MR Abdellatif2; Enas Abd El- Tawab Hassan1 | ||
| 1Agricultural Microbiology Dept, Fac of Agric, Ain Shames Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt | ||
| 2Agricultural Botany Dept, Fac of Agric, Ain Shames Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt | ||
| Abstract | ||
| Forty-six bacterial isolates were obtained from seven rhizosphere samples using nutrient agar and glucose agar media. These isolates were morphologically categorized into long rods, short rods, and cocci, comprising 52%, 17%, and 31% of the total count, respectively. The collected isolates from the rhizosphere were assessed for their capability to produce indole-3-acetic acid (IAA). The initial qualitative screening revealed diverse abilities among isolates for IAA production, with notable variations in productivity levels. Quantitative analysis of the top ten isolates revealed that cocci-shaped isolates produced the highest IAA levels (4.80 to 6.30 mg/100 mL), with the SS1 isolate achieving the maximum value (6.30 mg/100 mL). Genetic identification of the highest IAA producer (SS1) through 16S rRNA gene sequencing showed that it belongs to the genus Kocuria. Phylogenomic analysis confirmed a high similarity to Kocuria rosea, with 98% identity. | ||
| Keywords | ||
| Indoles; Rhizosphere; Kocuria; PGPR; 16S rRNA | ||
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