Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic Wistar rats: role of glucagon-like peptide-1
Nooman, M., Al-Kashef, A., Khattab, A., Ahmed, K., Rashad, M., Mahmoud, M. (2025). Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic Wistar rats: role of glucagon-like peptide-1. EKB Journal Management System, 24(1), 29-45. doi: 10.21608/epj.2025.402442
Mohamed U. Nooman; Amr S. Al-Kashef; Abd El-Nasser Khattab; Kawkab A. Ahmed; Mona M. Rashad; Maha H. Mahmoud. "Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic Wistar rats: role of glucagon-like peptide-1". EKB Journal Management System, 24, 1, 2025, 29-45. doi: 10.21608/epj.2025.402442
Nooman, M., Al-Kashef, A., Khattab, A., Ahmed, K., Rashad, M., Mahmoud, M. (2025). 'Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic Wistar rats: role of glucagon-like peptide-1', EKB Journal Management System, 24(1), pp. 29-45. doi: 10.21608/epj.2025.402442
Nooman, M., Al-Kashef, A., Khattab, A., Ahmed, K., Rashad, M., Mahmoud, M. Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic Wistar rats: role of glucagon-like peptide-1. EKB Journal Management System, 2025; 24(1): 29-45. doi: 10.21608/epj.2025.402442

Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic Wistar rats: role of glucagon-like peptide-1

Egyptian Pharmaceutical Journal
Article 4, Volume 24, Issue 1, January 2025, Page 29-45  XML PDF (12.51 MB)
DOI: 10.21608/epj.2025.402442
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Authors
Mohamed U. Nooman1; Amr S. Al-Kashef1; Abd El-Nasser Khattab2; Kawkab A. Ahmed3; Mona M. Rashad1; Maha H. Mahmoud4
1Department of aBiochemistry, Biotechnology Research Institute, National Research Centre, Cairo, Egypt
2Department of Genetics and Cytology, Biotechnology Research Institute, National Research Centre,, Cairo, Egypt
3Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
4Department of Nutrition and Food Science, Food Industries and Nutrition Research Institute, National Research Centre, Cairo, Egypt
Abstract
Background
One of the top ten causes of mortality all over the world is diabetes mellitus. Egypt in
particular, has one of the highest prevalence of diabetes mellitus in the world
Objective
The purpose of this work is to produce sophorolipids (SLs) economically through
microbial conversion of agro-industrial wastes namely Moringa oleifera (MO) and
Lepidium sativum (LS) oil cakes using locally isolated Saccharomyces cerevisiae
and Yarrowia lipolytica, respectively. Finally, the produced SLs will be investigated
for their potential use as antihyperglycemic drugs in diabetic rats.
Patients and methods
The SLs were structurally characterized by fourier transform infrared spectroscopy,
hydrogen-1 proton nuclear magnetic resonance, and LC-MS/MS. Then the
hypoglycemic impact of the produced SLs was evaluated through an experiment
on 30 male Wistar albino rats grouped into four diabetic groups induced by a single
intraperitoneal dose of streptozotocin; a control positive group, a diabetic
group + reference drug (metformin), a diabetic group + MO-SLs and a diabetic
group + LS-SLs and the experiment lasted for 4 weeks.
Results and conclusion
The yield of MO-SLs was 18.6 g/100 g substrate. While LS-SLs had a higher yield
(43.6 g/100 g substrate).TheMO-SLs declined surface tensionofwater to38mN/mat
60 mg/Lcriticalmicelleconcentrationhowever,LS-SLslevelswere46mN/mat50 mg/
L. The chemical characterization of MO-SLs indicated the presence of lactonic and
acidic formsofSLs and theLS-SLs confirmed the presence of lactonic formonly.Data
obtainedfor thehypoglycemic effect revealedthat bothextractshaveapositiveimpact
on the studied biochemical parameters with the best results recorded for body weight
change, fasting blood glucose, alanine transaminase, insulin, and glucagon-like
peptide-1 for the diabetic group receiving LS-SLs followed by the reference drug,
then the MO-SLs group. The results of biochemical markers were confirmed by the
histopathological examinations showing the efficiency of the produced LS-SLs in
decreasing the fasting blood glucose level.
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