IN VITRO: INHIBITION OF PARTIALLY PURIFIED PANCREATIC OVINE LIPASE BY WILLOW BARK EXTRACTS | ||||
| Journal of Bioscience and Applied Research | ||||
| Volume 11, Issue 1, March 2025, Page 168-179 PDF (1.15 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2025.339535.1113 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Fatima Murhaf Mohammed Alrushdi1; Omar Younis Mohammed Al-Abaasy2; Ruaa Nashat Al-Saffar3; Hadi Yasir Abbood4; Ahmed Khudhair Al-Hamairy5; Mohanad Yakdhan Saleh6; Heba Abdelzaher7; Mohamed Abuelseoud Abdelzaher   8; Mahmoud A. Kenawy9
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| 1College of Education for Pure Science, University of Mosul, Department of Chemistry.Mosul, Iraq. | ||||
| 23 Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq. | ||||
| 3Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq | ||||
| 4Medical Laboratories Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq. | ||||
| 5Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq. | ||||
| 6Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq. | ||||
| 7Department of Clinical pharmacy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt. | ||||
| 8Environmental Science and Industrial Development Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt. | ||||
| 9Biophysics Branch, Department of Physics, Faculty of Science, Al Azhar University, Nasr City 11884, Cairo, Egypt./ Radiology Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq | ||||
| Abstract | ||||
| Obesity has existed for a very long time. Obesity leads to improper physiological metabolism, which in turn produces a host of physiological and social issues in addition to aesthetic concerns. Individuals are working to discover anti-obesity medications and other safe and efficient treatment options. Because pancreatic lipase (PL) is essential to human fat metabolism, PL inhibitors are now used to treat obesity in clinical settings. This research involved partially purifying lipase from the ovine pancreas. One lipase isoenzyme was identified using the CM-Cellulose ion exchange chromatography technology. It had a specific activity of 2.467 U/mg protein and a recovery of 272 in comparison to the crude extract. The molecular weight was determined to be 33.8 kilodaltons, and the electrophoresis method produced only one band. Using p-nitrophenyl acetate as a substrate, the capacity of willow bark extracts to inhibit partly purified pancreatic lipase activity was investigated. The inhibitory percentages via treating the enzyme with EthOH 70% and H2O were found to be 27.14 and 61.43 respectively. Furthermore, the Lineweaver-Burk plots revealed the inhibition modes noncompetitive with both extracts. | ||||
| Keywords | ||||
| Ovine Pancreatic Lipase; Willow bark; Extracts; Purification; Inhibition | ||||
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