Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes
Elneely, E., Saad, E., Elsharkawy, A., Toson, E., Attia, Z. (2025). Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes. EKB Journal Management System, 68(8), 363-372. doi: 10.21608/ejchem.2025.327552.10618
Enas A. Elneely; Entsar A. Saad; Ashraf A. Elsharkawy; Elshahat A. Toson; Zeinab R. Attia. "Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes". EKB Journal Management System, 68, 8, 2025, 363-372. doi: 10.21608/ejchem.2025.327552.10618
Elneely, E., Saad, E., Elsharkawy, A., Toson, E., Attia, Z. (2025). 'Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes', EKB Journal Management System, 68(8), pp. 363-372. doi: 10.21608/ejchem.2025.327552.10618
Elneely, E., Saad, E., Elsharkawy, A., Toson, E., Attia, Z. Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes. EKB Journal Management System, 2025; 68(8): 363-372. doi: 10.21608/ejchem.2025.327552.10618

Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes

Egyptian Journal of Chemistry
Volume 68, Issue 8, August 2025, Page 363-372  XML PDF (557.69 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2025.327552.10618
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Authors
Enas A. Elneely1; Entsar A. Saadorcid 1; Ashraf A. Elsharkawy2; Elshahat A. Toson1; Zeinab R. Attia email orcid 3
1Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt
2Department of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt
3Mansoura University Children’s Hospital, Mansoura University, Mansoura, Egypt
Abstract
Abstract

T1DM is a long-term autoimmune condition impacted by both hereditary and environmental factors. Hyperglycemia encourages oxidative stress creation and activation of multiprotein inflammatory inflammasomes such as NLRP3. We aimed to assess and explain the relationships between the NLRP3 gene's rs10754558 C/G SNP, oxidative stress, and the severity of T1DM in Egyptian pediatrics. 120 T1DM patients and 121 age- and sex-matched healthy participants were included. Real-time PCR was carried out using TaqManTM SNP on the Artus Rotor-Gene Qiagen apparatus for genotyping. Malondialdehyde (MDA) and total antioxidant capacity (TAC) were estimated. Significant elevation in MDA (p<0.001) and reduction in TAC (p<0.001) were noticed. Glycosylated Hemoglobin (HbA1c) was negatively correlated with TAC among the T1DM group (r=-0.313, p<0.001). Serum MDA, TAC, and creatinine all showed elevated risk for predicting T1DM susceptibility, according to logistic regression analysis. The genetic variant of rs10754558 C/G in NLRP3 did not significantly correlate with T1DM susceptibility or MDA or TAC (p>0.05). Among T1DM patients, we confirm MDA elevation and TAC lowering as independent predictors for T1DM susceptibility. Moreover, NLRP3 rs10754558 C/G polymorphism may not be related to oxidative stress markers and disease susceptibility.
Keywords
T1DM; NLRP3; oxidative stress; genotyping; HbA1c
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