Establishment of hairy root cultures by Agrobacterium rhizogenes mediated transformation of Gardenia jasminoides Variegata for enhancing of phenolic and antioxidant accumulation capacity | ||||
| Egyptian Pharmaceutical Journal | ||||
| Article 12, Volume 24, Issue 1, January 2025, Page 131-140 PDF (2.07 MB) | ||||
| DOI: 10.21608/epj.2025.402973 | ||||
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| Authors | ||||
Hanan S. Ebrahim  1; Amal A. El Ashry1; Sally A.A. Rabie2; Ismail A. Ismail3; Mohamed K. El-Bahr4; Ahmed M.M. Gabr 5, 6
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| 1Departments of Plant Biotechnology, Biotechnology Research Institute, National Research Centre, Academy of Scientific Research and Technology, Cairo | ||||
| 2Departments of Genetics and Cytology, Biotechnology Research Institute, National Research Centre, Academy of Scientific Research and Technology, Cairo | ||||
| 3Plant genetic transformation department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, Giza, Egypt | ||||
| 4Departments of Plant Biotechnology, Biotechnology Research Institute, National Research Centre, Academy of Scientific Research and Technology, Cairo, | ||||
| 5Departments of Plant Biotechnology. Biotechnology Research Institute, National Research Centre, Academy of Scientific Research and Technology, Cairo | ||||
| 6Departments of Specialized Scientific Councils, Academy of Scientific Research and Technology, Cairo | ||||
| Abstract | ||||
| The gardenia is an aromatic medicinal plant belonging to the coffee family (Rubiaceae). Because of its high concentration of phenolic compounds, including caffeic acid, ferulic acid, and chlorogenic acid, it can be used to treat inflammatory illnesses and relieve pain. Objective It is the first study on Gardenia jasminoides Variegata hairy root cultures to increase the levels of Phenolic, flavonoid compounds, and accumulation of antioxidants. Patients and methods Gardenia jasminoides Variegata shoots were infected with the Agrobacterium rhizogenes A4 strain. Total phenolics and flavonoid content has been estimated. The content of phenolic and flavonoid compounds in the cultures was quantified using high-performance liquid chromatography. To emphasize and clarify the results the high-performance thin-layer chromatography were used. Antioxidant activity were demonstrated by 2,2′- azino-bis (3-ethylbenzothiazoline-6-sulfonate) radical cation and 2,2-Diphenyl-1-picrylhydrazyl. Results and conclusion The transgenic culture was confirmed through PCR using rol genes primers. Total phenolics content has been reported to be higher in hairy roots than in control samples recorded (1.674 as versus 1.073 mg/g DW, respectively). Additionally, the total flavonoid content in the transgenic stem cultures showed higher levels than the nontransgenics (2.824 versus 1.553 mg/g dry weight, respectively). Hairy root cultures recorded more antioxidant activity than the nontransgenics. Chlorogenic acid, ferulic acid, and caffeic acid were accumulated in high values in transgenic cultures (3248.4, 2948.2, and 452.2 μg/g DW, respectively). So, transgenic culture can accumulate as more as about 100 folds the nontransgenic ones for the chlorogenic acid and ferulic acid. | ||||
| Keywords | ||||
| Gardenia jasminoides Variegata; Agrobacterium rhizogenes mediated-transformation; Secondary metabolites; HPLC; HPTLC | ||||
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