Ameliorative effect of vitamin C and melatonin on the pancreatic toxicity induced by sodium glutamate in adult male albino rat: histological and immunohistochemical study | ||||
| Minia Journal of Medical Research | ||||
| Articles in Press, Accepted Manuscript, Available Online from 06 January 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjmr.2025.346151.1855 | ||||
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| Authors | ||||
Amira Moatamed Mahmoud  1; Abdel Hamid Sayed Abo Bakr Ali2; Medhat Atta3; Sayed Fouad El-sheikh Ali1
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| 1Human Anatomy and Embryology Department, Minia University, Minia | ||||
| 2Assistant professor of Anatomy and Embryology, Faculty of Medicine, Minia University, Egypt | ||||
| 3Assistant professor of Anatomy and Embryology Faculty of Medicine, Minia University | ||||
| Abstract | ||||
| ABSTRACT Background: Monosodium Glutamate (MSG) is a common flavor enhancer that has several toxic effects on different body organs. Vitamin C and melatonin are antioxidant agents with several pharmacological properties. Aim: The present study was conducted to clarify the possible protective role of vitamin C & melatonin on MSG-induced pancreatic toxicity. Materials and Methods: 50 adult male albino rats were divided into 5 equal groups. Group I was the control group, group II received MSG intraperitonially (4 mg/kg/day) for 14 days, group III received MSG as the same previous dose + vit C (500 mg/kg/day) for 14 days, group IV received MSG + melatonin (10mg/kg /day) for 14 days and group Ⅴ received MSG + vit C + melatonin as the same previous doses IP for 14 days. At the end of the experiment, specimens from the pancreas were taken and prepared for H&E, PAS and Mallory staining, immunohistochemical stain and biochemical analysis. Results: MSG increased the levels of pancreatic enzymes, as well as blood glucose levels. Insulin level was decreased. Histologically, degenerative changes were observed in pancreas with destruction in the pancreatic architecture as well as congestion and dilation of the blood vessels. There were signs of apoptosis. Co-administration of vit C & melatonin with MSG attenuated these degenerative effects. Conclusion: MSG caused deterioration of the pancreatic chemistry and architecture. Vit C & melatonin can improve these effects and the combination of these two supplements produced more favorable effects. | ||||
| Keywords | ||||
| Monosodium glutamate; vitamin C; melatonin; Pancreas | ||||
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