Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE | ||
| Parasitologists United Journal | ||
| Article 9, Volume 17, Issue 3, December 2024, Pages 211-213 PDF (317.65 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/puj.2024.313940.1265 | ||
| Authors | ||
| Zubaria Amin1; Muhammad Imran2; Asim Shamim* 3; Muhammad Sajid4; Hafiz Rizwan5; Tanzeel Rehman6; Syed Shah7 | ||
| 1Departments of Livestock and Dairy Development, Animal Science Institute, Manager Production, Hi Tech Feed and Oil Mills, Quetta, Balochistan | ||
| 2Departments of Parasitology, Faculty of Veterinary Science, University of Agriculture ,Manager Production, Hi Tech Feed and Oil Mills, Quetta, Faisalabad | ||
| 3Faculty of Veterinary and Animal Sciences , Manager Production, Hi Tech Feed and Oil Mills, Azad Kashmir3, Pakistan | ||
| 4University of Agriculture , Manager Production, Hi Tech Feed and Oil Mills, Quetta Faisalabad ,Pakistan | ||
| 5Manager Production, Hi Tech Feed and Oil Mills, Quetta , Lahore ,Pakistan | ||
| 6Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Sahiwal , Pakistan | ||
| 7Faculty of Veterinary and Animal Sciences, University of Poonch , Pathobiology (Parasitology)section, Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Rawalakot, Azad Kashmir , Pakistan | ||
| Abstract | ||
| Background: Helminthic excretory secretory (E/S) antigens gained considerable interest due to their role in stimulating host-parasite interactions and as potential candidate for vaccine development. Objective: To quantify and characterize H. contortus E/S antigens aiming for its use as an effective control measure. Material and Methods: Adult worms were collected from the abomasa of slaughtered goats. Fresh and highly motile adult worms were transferred to RPMI 1640 culture medium containing antibiotics. The E/S antigen was procured through standard protocols and concentration of proteins was measured. Characterization of ES antigen was subjected to SDS-PAGE and estimation of protein concentration. Results: The triturate contained proteins with a concentration of 0.836 mg/ml. Three bands of proteins appeared on the gel with molecular weights of 65, 48 and 30 kDa. Conclusion: Further characterization of E/S antigens will be helpful in developing effective control measures against the worms in general and haemonchosis specifically. | ||
| Keywords | ||
| E/S antigens; goats; H. contortus; immunodiagnosis; nematodes; SDS-PAGE | ||
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