Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice
Shehata, M., El Naggar, H., Abdelmaksoud, H., El-Ashkar, A., Barakat, A., Abdelhameed, R., Atta, S., Mohammad, O. (2024). Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice. EKB Journal Management System, 17(3), 240-246. doi: 10.21608/puj.2024.331310.1275
Mai Shehata; Heba El Naggar; Hagar Abdelmaksoud; Ayman El-Ashkar; Ashraf Barakat; Reda Abdelhameed; Shimaa Atta; Omnia Mohammad. "Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice". EKB Journal Management System, 17, 3, 2024, 240-246. doi: 10.21608/puj.2024.331310.1275
Shehata, M., El Naggar, H., Abdelmaksoud, H., El-Ashkar, A., Barakat, A., Abdelhameed, R., Atta, S., Mohammad, O. (2024). 'Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice', EKB Journal Management System, 17(3), pp. 240-246. doi: 10.21608/puj.2024.331310.1275
Shehata, M., El Naggar, H., Abdelmaksoud, H., El-Ashkar, A., Barakat, A., Abdelhameed, R., Atta, S., Mohammad, O. Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice. EKB Journal Management System, 2024; 17(3): 240-246. doi: 10.21608/puj.2024.331310.1275

Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice

Parasitologists United Journal
Article 14, Volume 17, Issue 3, December 2024, Page 240-246  XML PDF (571.05 K)
Document Type: Original Article
DOI: 10.21608/puj.2024.331310.1275
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Authors
Mai Shehata email 1; Heba El Naggarorcid 1; Hagar Abdelmaksoudorcid 2; Ayman El-Ashkar3; Ashraf Barakatorcid 4; Reda Abdelhameed5; Shimaa Atta6; Omnia Mohammad1
1Departments of Medical Parasitology, Faculty of Medicine, Ain Shams University, Cairo
2Theodor Bilharz Research Institute, Giza
3Departments of Medical Parasitology, Faculty of Medicine, Ain Shams University, Cairo & Microorganisms and Clinical Parasitology, College of Medicine, University of Bisha, Bisha, Saudi Arabia
4Zoonoti Diseases , National Research Centre, Giza , Egypt
5Applied Organic Chemistry, Chemical Industries Research Institute National Research Centre
6Theodor Bilharz Research Institute, Giza , Egypt
Abstract
Background: Although several drugs and natural products were investigated in the treatment of chronic
toxoplasmosis, brain cysts are still resistant to eradication.
Objective: To evaluate the immunomodulatory effect of Nigella sativa oil (NSO) and wheat germ oil (WGO)
loaded metal-organic frameworks (MOF) on chronic toxoplasmosis in mice.
Material and Methods: Eighty male Swiss albino mice were divided into 8 groups (10 mice each); GI: noninfected
non treated, GII-GVIII: infected with ME-49 avirulent strain of T. gondii; GII: non-treated, GIII-GVIII
were treated with Spiramycin (GIII), Spiramycin-MOF (GIV), MOF (GV), WGO-MOF (GVI), NSO-MOF (GVII),
and WGO+NSO-MOF (GVIII). Immunological evaluation was performed through immunohistochemical
(IHC) staining of liver sections, and serological detection of immunological markers, i.e., interferon-gamma
(INF–γ), and tumor necrosis factor-alpha (TNF–α).
Results: The IHC examination revealed a high predominance of CD8+ cells elicited in GVII, and GVIII
followed by GVI. A statistically significant difference was recorded between the treated test and control
groups regarding the levels of INF–γ, and TNF–α in their sera with the highest level recorded in GVII
followed by GVIII, GVI, GIII, GIV, and GV, respectively.
Conclusion: Our study showed that NSO and WGO combinations with MOF exhibited significant
immunostimulant effects against chronic murine toxoplasmosis.
Keywords
essential oils; metal-organic framework; Nigella sativa; spiramycin; Toxoplasma; wheat germ
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