Histological and Histochemical Investigations on the Toxic Effects of Acrylamide and Ameliorative Effects Using Moringa oleifera Leave Nanoparticles on the Liver, Kidney, and Testis of Adult Male Rats. | ||
| Scientific Journal for Damietta Faculty of Science | ||
| Volume 14, Issue 3, December 2024, Pages 76-89 PDF (1.09 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/sjdfs.2025.327142.1185 | ||
| Authors | ||
| Abdullah Hamad Yaquob Abduljalil* 1; Kadry Elbakry2; Nahed Omar3; Lamiaa Deef4; shereen Ahmed Fahmy5 | ||
| 1Zoology Department , Faculty of Science ,Damietta University | ||
| 2Zoology Dep. Faculty of Science, Damietta University | ||
| 3Emeritus Associate Professor of Histology & Histochemsitry, Department of Zoology, Faculty of Science, Damietta University | ||
| 4Assistant Professor of Molecular Biology, Department of Zoology, Faculty of Science, Damietta University | ||
| 5Assistant Professor of Parasitology. Department of Zoology . Faculty of Science , Damietta University , Egypt | ||
| Abstract | ||
| The present study was conducted to evaluate the toxic effects resulted from acrylamide (ACR) and the protective effects of Moringa oleifera (M. oleifera) nanoparticles to adult male rats. Twenty adult male rats were randomly divided into four groups: Group I (Control group); Group II (Acrylamide group): rat received 50 mg/kg b.wt. of ACR in drinking water for 3 weeks; Group III (M. oleifera nanoparticles group): rats received 50 mg/kg b.wt of M. oleifera nanoparticles daily for 3 weeks; and Group IV (M. oleifera nanoparticles + Acrylamide group): rats were given 50 mg/kg b.wt of ACR and received M. oleifera nanoparticles 50 mg/kg b.wt orally, daily, at the same time for 3 weeks. Blood and tissue samples were collected for the physiological histological, and histochemical studies. The histological study revealed that rats exposed to ACR had significant damage to their kidneys, livers, and testicles, along with inflammatory changes and structural deformities. Treatment with M. oleifera nanoparticles improve histopathological changes and reduced susceptibility to ACR toxicity in adult male rats. Three-week treatment of M. oleifera nanoparticles largely recover the reduction in proteins and carbohydrates observed in ACR administration. The application of M. oleifera nanoparticles with ACR significantly reduce the histochemical damage that caused by ACR. The treatment also reduce ACR's destructive impact on activities of ALT, AST enzymes, and MDA, creatinine, and urea levels while increase SOD enzyme activity. From the results one can suggest that M. oleifera nanoparticles is a potential cytoprotective agent against ACR's pathological toxicity. | ||
| Keywords | ||
| ACR; M.oleifera; Physiology; Histology; Histochemistry | ||
|
Statistics Article View: 164 PDF Download: 111 |
||
