Estimation of Postmortem Interval Using Various Markers in the Liver of Adult Albino Rats (Targeting the PTEN/FasL Signaling Pathway) | ||||
| The Egyptian Journal of Forensic Sciences and Applied Toxicology | ||||
| Volume 24, Issue 4, December 2024, Page 53-70 PDF (1.55 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejfsat.2024.315882.1347 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Rabab fawzy Hindawy1; EMAN FARUK2; samia Mahmoud Manawy3; Basma A. Ibrahim4; Doaa Elsayed Abdelrazzak5; Nahla M Ibrahim   6
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| 1forensic medicine and clinical toxicology department, faculty of medicine, benha university, benha, egypt | ||||
| 2Department of Histology and Cell Biology, Faculty of Medicine, Banha University, Banha 13518, Egypt | ||||
| 3: assistant professor anatomy and embryology Benha faculty of medicine | ||||
| 4Lecturer in Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt | ||||
| 5forensic medicine and clinical toxicology department, faculty of medicine, zagazig university, egypt | ||||
| 6Lecturer of Forensic medicine and Toxicology Zagazig University | ||||
| Abstract | ||||
| Estimation of time since death is one of the challenges in forensic science. Autolysis triggers decomposition, which induces cellular destructive changes causing cell death. Aim: This work aimed to study mRNA expression based on the process of cell death signaling, to evaluate changes in oxidant/ antioxidant parameters and immunohistochemical postmortem changes in the rat's liver. Methodology: 35 rats were divided into 5 groups. The liver was extracted at (0, 3, 6, 12, 24) hours postmortem, to measure the mRNA expression of (FasL) and (PTEN) by quantitative PCR in addition to MDA, CAT, GPx, and immunohistochemical postmortem changes. Results: MDA began to rise noticeably after death, CAT began to decline at 3 hours, GPx declined at 6 hours. The relative expression of FasL gene in the rat's liver increased at 3, 6, 12, and 24 h. However, at 24 h pm, it suddenly decreased. Also, the relative expression of the PTEN gene is increased from 3 to 12 h and then decreased at 24 h postmortem. Regarding mRNA levels of caspase-3 and Bax, there was a time-dependent rise till 12 h and a noticeable decline at 24 h. Oppositely the relative expression of the Bcl2 gene decreased from 3 to 24 h postmortem. The relative mRNA expression of FasL, PTEN, caspase 3, and Bax showed a strong positive correlation with the early postmortem time. The relative expression of the Bcl2 gene was found to correlate negatively with the early postmortem time. Conclusion: These findings supported our preliminary theory, since FasL, PTEN, caspase 3, Bax, and Bcl2 are involved in necrobiosis signaling mechanisms. They are potential candidates to analyze the time since death in 24h or less intervals. The oxidant/antioxidant and immunohistochemical changes in the liver of rats are also important to determine the time after death. It is possible to estimate the early postmortem interval using these biochemical markers. | ||||
| Keywords | ||||
| Keywords: postmortem interval; biomarkers; liver; FasL; PTEN; and adult albino rats | ||||
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