Modified combined disc test versus combined disc test in detection of carbapenemase producing Klebsiella pneumoniae at a tertiary care hospital, Egypt. | ||
| Microbes and Infectious Diseases | ||
| Volume 6, Issue 4, November 2025, Pages 6615-6626 PDF (566.77 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.341121.2379 | ||
| Authors | ||
| Alshimaa L. Abdallah* 1; Aref M. Maarouf2; Aya A. Al-Baz1; Ayman M. Marei1 | ||
| 1Medical Microbiology and Immunology Department, faculty of medicine, Zagazig University, Egypt | ||
| 2Urology Department, faculty of medicine, Zagazig University, Egypt | ||
| Abstract | ||
| Background: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is considered a distressing healthcare problem owing to limited choices in its therapy. This study aimed to evaluate mCDT for carbapenem’s detection in (Kp) in comparison to the traditional CDT and comparing their sensitivity and specificity. Methods: Thirty-six carbapenemase producer Klebsiella pneumoniae were enrolled in our study, they were previously isolated, conventionally identified and tested for carbapenemase production by screening method in other previous study, they were confirmed and differentiated into classes by combined disc test (CDT) and polymerase Chain reaction (PCR). The PCR positive strains were finally subjected to mCDT. Results: Thirty-five of the 36 CR-Kp isolates were positive for CDT and mCDT. mCDT with sensitivity and specificity to be 95.5% and 100%, respectively regarding Class B carbapenemase detection. Whereas Class D carbapenemase sensitivity and specificity of this modification, were 77.8% and 100%, respectively. CDT and mCDT were fully agreed when compared with PCR results. Conclusion: mCDT is a sensitive and specific phenotypic method with the same accuracy as PCR. | ||
| Keywords | ||
| Carbapenem-resistant Klebsiella pneumoniae; mCDT; CDT; antibiotic resistance; phenotypic detection | ||
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