Correlation between Methicillin Resistance Gene Presence, Biofilm Production and Antibiotics Pattern of Staphylococcus aureus Wound Isolates and the Activity of Possible River Water Bacteriophage Against Them | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Article 20, Volume 34, Issue 2, April 2025, Page 175-181 PDF (478.3 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2025.341642.1393 | ||||
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| Authors | ||||
Sarah A.H. Abdulsattar  1; Mohammed J. Alfeehan2; Mohammed R. Mohaisen 3
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| 1Microbiology Department, College of Medicine, University of Anbar, Ramadi, Iraq | ||||
| 2Surgery Department, College of Medicine, University of Anbar, Ramadi, Iraq | ||||
| 3Department of Basic Sciences, College of Dentistry, University of Anbar, Ramadi, Iraq; Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, L69 7TX, United Kingdom. | ||||
| Abstract | ||||
| Background: Wound infections with Staphylococcus aureus strains, a potential health care problem, especially after methicillin-resistant Staphylococcus aureus (MRSA) occurrence, which poses a significant challenge for healthcare workers. Objective: is to identify the relationship between the existence of the gene mecA within isolated bacterial cells and the phenomenon of multiple antibiotic resistance profiles of these strains. The study also explored the potential therapeutic activity of river water bacteriophage. Methodology: Among other bacterial species isolated from wound infections, 34 strains of S. aureus were isolated. After the screening for mecA gene using the Polymerase Chain Reaction Technique (PCR), the antibiotic susceptibility profiles of S. aureus isolates were determined by the disk diffusion method to identify the relationship between the two parameters. Furthermore, the possibility of Bacteriophage existence against isolated S. aureus was investigated. Results: A strong correlation between the existence of the gene mecA and the phenomenon of multiple antibiotic resistance profiles, particularly beta-lactams as well as commonly prescribed antimicrobial agents was present. Moreover, the results of the bacteriophage spots showed the possible presence of bacteriophage against S. aureus wound isolates. Conclusion: There was significant relationship between bacterial possessing of gene mecA, their biofilm production ability and their multidrug resistant phenomenon. River water bacteriophage could be used to treat pathogen bacteria included in this study. | ||||
| Keywords | ||||
| MRSA; Antibiotics; mecA genes; Staphylococcal aureus; wound infections | ||||
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