Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation | ||
| Egyptian Academic Journal of Biological Sciences, B. Zoology | ||
| Volume 17, Issue 1, June 2025, Pages 15-25 PDF (915.46 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsz.2025.404827 | ||
| Authors | ||
| Ramy B. Ibrahim1; Hala Gabr2; Fadia M. Atia3; Aida A. Hussein1 | ||
| 1Zoology department, Faculty of Science, Suez University, Egypt. | ||
| 2Hematology department, Faculty of Medicine, Cairo University, Egypt. | ||
| 3Clinical Pathology department Faculty of Medicine, Suez Canal University, Egypt. | ||
| Abstract | ||
| Aacute myeloid leukemia (AML) is a severe hematological malignancy with a poor prognosis. Although chemotherapy remains the standard treatment, its effectiveness is limited, with high rates of relapse. Stem cell therapy has emerged as a promising alternative, yet its potential for AML prevention and treatment requires further investigation. This study aimed to evaluate the efficacy of bone marrow-derived mesenchymal stem cells (BM-MSCs) in promoting the maturation of AML blasts. Peripheral blood samples were obtained from 39 AML patients for the isolation of blast cells, while BM-MSCs were derived from healthy donors. An in vitro co-culture system was employed to study the interactions between BM-MSCs and AML blasts, assessing the proliferative capacity of BM-MSCs and their effects on leukemia cells (LCs). Flow cytometry was used to evaluate the expression of specific markers before and after co-culture with allogeneic BM-MSCs, focusing on the induction of AML blast maturation. The results demonstrated a significant reduction in the expression of CD13 and CD33 markers in AML blasts following co-culture with BM-MSCs across all samples. Specifically, the expression of CD13 decreased from 70.74% to 5.02%, and CD33 expression was reduced from 69.49% to 5.14%, indicating a marked decline in AML blast populations. In conclusion, the findings suggest that BM-MSCs appear to be effective in inducing the maturation of AML blasts, offering a potential therapeutic strategy for AML management. | ||
| Keywords | ||
| Acute myeloid leukaemia (AML); Bone marrow derived Mesenchymal stem cells (BM-MSCs); leukemia cells (LCs) | ||
|
Statistics Article View: 178 PDF Download: 184 |
||
