Incidence of resistance pattern and biofilm-forming genes of Acinetobacter baumannii isolated from Iraqi children | ||
| Microbes and Infectious Diseases | ||
| Volume 6, Issue 4, November 2025, Pages 6668-6676 PDF (634.98 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.348347.2417 | ||
| Authors | ||
| Firas Nabeeh Jaafar1; Hamzah Abdulrahman Salman* 2; Shatha Mahmood Ali3; Zainab Mohsin Ahmed4 | ||
| 1Microbiology Department, College of Science, Mustansiriyah University, Baghdad, Iraq | ||
| 2Department of Medical Laboratory Techniques, Technical Institute of Balad, Middle Technical University, Baghdad, Iraq | ||
| 3Imamein Kadhimein Medical city, Baghdad, Iraq. | ||
| 4Central Teaching Hospital of Pediatrics in Baghdad, Iraq | ||
| Abstract | ||
| Background: Acinetobacter baumannii is frequently implicated in nosocomial infections, causing a diverse range of illnesses in humankind. The objective of the study was to investigate the multidrug-resistant and biofilm-forming A. baumannii isolated from Iraqi children. Methods: Seventy-five blood and urine samples were collected from children in Baghdad, Iraq. VITEK 2 compact was used for identification and antibiotic susceptibility testing. Biofilm production was assessed using microtiter plates, and biofilm genes such as csuE, pgaB, bfmS, and ompA were detected using conventional PCR. Results: Of the sample size, 54 isolates were identified as A. baumannii. Among the tested antibiotics, ticarcillin (68.5%) showed the highest resistance rate, while colistin showed the highest susceptibility rate (92.6%). Furthermore, 61.11% of the isolates were XDR, and 29.6% were MDR. All the isolates showed the capability for biofilm production; 63% and 37% of isolates produced robust and moderate biofilm, respectively. All the isolates were determined to contain a minimum of one gene relevant to biofilm formation; however, the most prevalent gene was csuE (100%). Conclusion: High resistance rates were shown among A. baumannii strains. Moreover, biofilm gene csuE was shown in all the strains. However, further experimentation to evaluate biofilms' involvement in the pathophysiology of A. baumannii infections is mandatory. | ||
| Keywords | ||
| A. baumannii; Biofilm; Antibacterial susceptibility; multidrug resistant; OmpA gene | ||
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