Detection of eno gene in methicillin-resistant Staphylococcus aureus. | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 14 January 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.328933.2292 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Noor Thaer Salih   1; Essra Ghanim Alsammak 2
| ||||
| 1Anesthesia Techniques, Northern Technical University, Mosul, Iraq | ||||
| 2Department of biology, Collage of science, university of Mosul, Mosul, Iraq | ||||
| Abstract | ||||
| Background: Staphylococcus aureus is a type of Gram-positive bacteria that naturally inhabits human and animal skin in addition to mucous membranes, often found in symbiosis with other bacteria. It can act as an opportunistic pathogen, causing diseases ranging from skin abscesses to severe conditions such as bacteremia and endocarditis due to its possession of several virulence factors. Thus, this study investigate the relationship between biofilm formation and presence of adhesion gene eno gene. Methods: Clinical and nasal samples were obtain from both sexes from Al Salam , Al Khansa hospitals and external lab , all samples were cultured on mannitol salt agar medium . Then, the capacity of the positive isolates to produce coagulase was examined. and the diagnoses assured by vitek2 system . also resistance to methicillin were tested using the antibiotic Oxacillin (methicillin) and MRSA isolates were detected. Results: From 50 clinical (pathogenic- from patient ) samples 25 of them grew on mannitol salt agar and 30 out of 50 nasal (from healthy people ) swabs showed growth on this medium too, and after performing the coagulase test, 14 clinical isolates and 8 nasal isolates showed as Staphylococcus spp. Resistance to some antibiotics such as penicillin was 100% for clinical and nasal isolates, and resistance to oxacillin (methicillin) by clinical isolates was (93%) while all the nasal isolates were sensitive to it. Also, the resistance of clinical isolates to vancomycin was (78.5%) but all nasal isolates were sensitive to vancomycin. The rate of presence of the eno gene was (93%), (100%) in clinical and nasal isolates respectively. eno gene was present in the nasal isolates that did not form biofilm on (CRA), while one of the clinical isolates didn’t contain eno gene that was positive for biofilm formation in both (CRA ) and (MTP) method. The relationship between Staph. aureus strains is shown in the phylogenetic tree based on the eno gene. Cluster A was linked together with B with 99.99% similarity, and they were also linked together with cluster C with 99.98%. This gene was registered in National Center for Biotechnology information (NCBI) with a special accession number for sex isolates. Conclusions: The prevalence of the eno gene did not differ between the isolates that formed and did not form a biofilm. Also, the great closeness in the sequences of the eno gene indicates the stability of this gene and its important role in diagnosis and classification. | ||||
| Keywords | ||||
| eno gene; MRSA; phylogenetic tree | ||||
|
Statistics Article View: 168 |
||||
