Histomorphometric characteristics and dystrophins localization in the hypothalamic supraoptic nucleus and hypophyseal neural lobe of Jaculus orientalis under arid conditions

Rouaiguia, Nadia; BENABDESSELAM, Roza; Souttou, sabrina; Ouhida, Soraya; Guendouzi, Salima; Dorbani-Mamine, Latifa

doi:10.21608/ejh.2025.329659.2161

	Histomorphometric characteristics and dystrophins localization in the hypothalamic supraoptic nucleus and hypophyseal neural lobe of Jaculus orientalis under arid conditions
Egyptian Journal of Histology
Articles in Press, Accepted Manuscript, Available Online from 16 January 2025
Document Type: Original Article
DOI: 10.21608/ejh.2025.329659.2161
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Authors
Nadia Rouaiguia ¹; Roza BENABDESSELAM ²; sabrina Souttou ²; Soraya Ouhida ³; Salima Guendouzi ²; Latifa Dorbani-Mamine ²
¹Neurochemistry Team, Faculty of Biological Sciences, Houari Boumediene Sciences and Technology University (FSB-USTHB), Algiers, Algeria
²Neurochemistry team, Faculty of Biological Sciences, Houari Boumediene Sciences Technology University (FSB/USTHB),
³Department of Medicine, Saadna Mohamed Abdennour University-Hospital Center, Setif, Algeria
Abstract
Introduction: The large Egyptian jerboa, Jaculus orientalis, is a xeric rodent characterized by its tolerance to prolonged dry seasons that can last several months. Aim of the Work: To clarify this biological characteristic, we evaluated the levels of osmolality, plasma electrolytes, and hematocrit in male Jaculus orientalis captured in the July. A histological study of the SON and NL/HP and an immunohistochemical study of the markers of morpho-functional plasticity; dystrophins and GFAP were carried out in these two structures. Materials and Methods: Twenty wild male Jaculus orientalis (120.9±2.25g) were employed in this study. After sacrifice, the blood of six male Jaculus orientalis was used for the determination of plasma parameters. The paraffin sections of SON and NL/HP were practiced for touluidine blue for topographic study. Immunohistochemical study of anti-dystrophins and anti-GFAP were carried out in these two structures. Studies employing morphometry and statistics were conducted as well. Results: In Jaculus orientalis captured in July, the hematocrit and plasma osmolality were higher than those previously reported in many rodents. Furthermore, SON and NL/HP showed changes in structural organization, such as the development of magnocellular neuron somas, hypervascularization, and high pituicyte density. In SON, Dps appears in astrocyte cell bodies and their retracted processes, identified with GFAP, as well as in the perinuclear and perimembrane of magnocellular neurons. In NL/HP, Dps are present around blood capillaries, pituitary cell bodies, and nerve endings. This distribution highlights the importance of Dps in the adaptation process of Jaculus orientalis to arid conditions through neurono-glio-vascular connections for the detection of central osmolality and neurosecretory response.
Keywords
Dystrophins; Jaculus-orientalis; supraoptic-nucleus; hypophysis; arid


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