Bacterial characterization and evaluation of IL6 and TLR-2 levels in diabetic foot infection | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 16 January 2025 PDF (902.66 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.338739.2363 | ||||
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| Author | ||||
Farah Ali Hameed 
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| Department of Emergency medicine and first aids Techniques, Institute of Medical Technology/ Baghdad, Middle Technical University, Baghdad, Iraq. | ||||
| Abstract | ||||
| Background: According to international Diabetes Federation Diabetes, diabetes lines among the top 10 reasons of mortality in the world. Objectives of the study: This study aimed to bacterial characterization and assess the levels of interlukin-6 (IL-6) and toll like receptor-2 (TLR-2) in diabetic foot infections. Methods: The study included 100 participants who were divided into two groups: 60 patients with diabetic foot infections who visited the outpatient clinic for diabetic foot patients in Baghdad and had a history of diabetes, while 40 healthy individuals served as a control group. Swabs from the ulcers were taken and underwent culture and microbiological identification. Antibiotic susceptibility testing was performed on the bacterial isolates. Venous blood samples were taken from the patients and controls, and ELISA technique was used to detect serum IL6 and TLR-2 levels. Results: The results showed that Gram-positive bacteria were more prevalent than gram negative bacteria. Most of the foot ulcer infected patients were older patients (50-60) years. There was an increased ciprofloxacin sensitivity to Staphylococcus aureus (S. aureus). The mean level of Toll-like receptor 2 (TLR-2) and IL-6 in diabetic foot infection patients was (6.99±1.90) compared to the control group (4.63 ±0.94), with a highly significant difference (p≤ 0.001). Conclusions: Klebsiella pneumoniae was the most commonly isolated Gram-negative bacteria, while S. aureus was the most common Gram-positive bacteria. There was a high rate of antibiotic resistance to K. pneumoniae and S. aureus. The current study revealed that there was a highly significant elevation in TLR-2 and IL-6 in diabetic patients with foot ulcers. | ||||
| Keywords | ||||
| Diabetic foot ulcer; bacterial isolate; IL-6; TLR-2 | ||||
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