Highly sensitive green micellar fluorometric method, assed by GABI, for determination of mosapride in pure form, dosage forms, and spiked human plasma.

Saraya, Roshdy elsayed; salman, baher ibrahim

doi:10.21608/odr.2025.351858.1047

	Highly sensitive green micellar fluorometric method, assed by GABI, for determination of mosapride in pure form, dosage forms, and spiked human plasma.
Octahedron Drug Research
Article 5, Volume 6, Issue 1, January 2025, Page 50-57 PDF (527.93 K)
Document Type: Research Articles
DOI: 10.21608/odr.2025.351858.1047
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Authors
Roshdy elsayed Saraya ¹; baher ibrahim salman ²
¹Analytical Chemistry Department, Faculty of Pharmacy, PortSaid University
²al azhar university faculty of pharmacy assiut branch
Abstract
The suggested analytical technique uses sensitive spectrofluorimetric method to determine Mosapride (MS) in pure form, pharmaceutical dosage forms, and spiked human plasma. MS is selective serotonin 5-HT4 agonist that is used in treatment of gastrointestinal motility impairment. To achieve the highest sensitivity, the method was adjusted for buffer type, pH, surfactant type, concentration, diluting solvent, and time. It relies on the enhancement of MS native florescence through micelle formation with anionic surfactant sodium dodecyl sulphate SDS, which increases the native florescence by 300%. The results demonstrated that, after a 10-minute reaction, the maximum sensitivity was achieved using SDS (10 mM) in acetate buffer (PH 6). This indicates the highest RFI for MS that can be measured at 352 nm following excitation at 319 nm. With a detection limit of 14.4 ng ml-1 and a quantitation limit of 43.2 ng ml-1 for MS, the calibration curves were linear spanning the 50–2000 ng ml-1 range. The findings of the suggested analytical method's validation in accordance with ICH requirements were deemed acceptable. The suggested approach yields a high recovery rate and has been effectively used to identify the medication under study in both its pharmaceutical forms and in human plasma that has been tampered with. The greenness of the proposed method was evaluated using GABI, and AGREE methods. The precision and accuracy of the results were in great accord with the specified approach.
Keywords
mosapride; micellar; fluorometry; sodium dodecyl sulphate; human plasma


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